【摘要】 目的 研究螺旋藻多糖对体外培养的人肝癌细胞株(HepG-2)细胞生长的影响。 方法 MTT法测定螺旋藻多糖的抗肿瘤活性;光镜观察螺旋藻多糖对HepG-2细胞形态学的影响;流式细胞仪检测螺旋藻多糖对HepG-2细胞周期的影响。 结果 随螺旋藻多糖浓度的增加，HepG-2细胞存活率逐渐降低，抑制率逐渐增加;光镜下观察显示，螺旋藻多糖作用24～48h后，细胞出现明显的形态学改变;流式细胞仪证实螺旋藻多糖对HepG-2细胞存在周期特异性，使细胞发生G 1 期阻滞。 结论 螺旋藻多糖可直接抑制HepG-2细胞的增殖。

    【关键词】 螺旋藻多糖 HepG-2细胞 细胞周期

    Antiproliferation effects of polysaccharides from spirulina platensis on HepG-2cells

    Yan Bomin，Yu Hong

    Department of General Surgery，Wanjie Hospital，Qingdao266071.

    【Abstract】 Objective To investigate the antiproliferation effects of polysaccharide from spirulina platensis(PSP)on HepG-2cells.Methods The antiproliferation activities of PSP were evaluated on HepG-2cells by MTT assay.The morphological changes were observed under light microscope.Cell division cycle was measured by flow cy-tometry.Results MTT assay showed that with the increase of PSP concentrations ......