关键词:丁公藤注射液;东莨菪内酯;药代动力学;血浆
摘 要: 用 PR-HPLC 法测定丁公藤注射液有效成分总东莨菪内酯在小鼠血浆中的浓度。色谱条件:SpheriSorb_C18 不锈钢柱,流动相为甲醇-水-冰醋酸(38∶62∶0.19),检测波长 345 nm。血浆样品经酸水解后用氯仿萃取,50℃水浴,氮气吹干,甲醇超声溶解后进样。本法具有快速、简便、灵敏、准确等特点,已经用于总东莨菪内酯血药浓度测定及药代动力学研究,结果满意。
分类号: R969.1 文献标识码: A
文章编号: 1003-9783(2000)01-0011-03
Determination of Plasma Concentration of Total Scopoletin by RP-HPLC
WEI Min, ZHOU Li-ing, LI Rui
( Guangzhou University of TCM, Guangzhou 510405)
Abstract: The plasma concentration of total scopoletin in Dinggongteng Injection was determined by RP_HPLC. The chromatographic conditions were as follows: stainless column Spherisorb C18 ; mobile phase:methanol_water_glacial acetic acid (38∶ 12∶ 0.19); detection wave length 345nm. The plasma sample was extracted by chloroform after acid hydrolysis, dried by nitrogen on a water bath at 50℃ , and dissolved with methanol. This method is rapid simple, sensitive and accurate, and has been used in plasma concentration determination and pharmacokinetics of total scopoletin.
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