刘宗印 孙玉萍 肖东杰 张红 李均 赵建强 孙善会 刘宗印(济南市中心医院 济南市 250013)；孙玉萍(济南市中心医院 济南市 250013)；肖东杰(济南市中心医院 济南市 250013)；张红(济南市中心医院 济南市 250013)；李均(济南市中心医院 济南市 250013) 中国肿瘤临床 2000 0 27 6


    关键词：干细胞；动员；冷存；实体瘤 期刊 zgzllc 0 论著 fur -->


    

摘要 目的：探讨一种适于晚期恶性肿瘤治疗的自体外周血造血干细胞移植方法。方法：单用rG-CSF(75μg,q12h×2d)作动员剂，用血细胞分离机一次性收集单个核细胞(MNC),4℃贮存。观察WBC、MNC计数、细胞活力及细胞分类；用流式细胞仪进行细胞表型检测。结果：小剂量rG-CSF动员后，外周血WBC达(18.71±6.54)×10⁹ /L。一次可收集MNC(1.87±0.66)×10⁸ /kg。CD₃₄ 、CD₁₄ 、CD₃ 及CD₂₂ 细胞分别为4.39%、3.85%、4.63%及3.76%。CD34细胞平均约为8.2×10⁶ /kg。4℃贮存48～56小时MNC存活率为94.3%。结论：对实体瘤尚无骨髓转移的患者，用此方法可获得足以重建造血功能的干细胞。

Mobilization and Preservation of Peripheral Stem Cells in Solid Tumors

Liu Zongyin Sun Yuping Xiao Dongjie et al

    (Jinan Central Hospital,Jinan)

Abstract Objective:To probe the way of autologous peripheral blood stem cell transplantation (APBSCT) for treatment of advanced malignant tumor.Methods:The peripheral blood stem cells were mobilized by rG-CSF (75ug q12h x 2d) and isolated by CS-3000 Plus Blood Cell Seperator and stored at 4℃ for 48～56 hrs.The number of WBC and MNC and cell vibility were counted and classified by BVPM multi-microscope and CD₃₄ ,CD₁₄ ,CD₃ and CD₂₂ were determined by flow microfluorometer.Results:After mobilization by G-CSF,the number of WBC increased to 18.71±6.54× 10⁹ /L (averagely 3.23 times of that before mobilized).MNC was 1.87±0.66×10⁹ Kg and CD34 cell number was 8.2× 10⁶ /Kg.The positive rates of CD₃₄ ,CD₁₄ ,CD₃ and CD₂₂ were 4.39%,3.85%,4.63% and 3.76% respectively.The survival rate of MNC at 4℃ was 94.3%.Conclusion:The results showed that mobilized APBSC stored at 4℃ is helpful for the patients with solid malignant tumor to get reconstructed hemopoietic function under high dose chemotherapy.

    Key Words Stem cell Mobilize Preservation Solid tumor

自1985年首例AML采用自体外周血造血干细胞移植(Autologous peripheral blood stem cell transplantation,APBSCT)获得成功后 ......

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