梁浩 广西医科大学流行病学教研室 南宁 530021 广西医科大学学报 1999 0 16 2


    关键词：聚合酶链反应；巨细胞病毒；献血员 期刊 gxykdxxb 0 论著 fur -->


    

摘要 目的 ：揭示献血员中巨细胞病毒(HCMV)感染状况，并探讨用聚合酶链反应(PCR)技术对献血员的血液进行HCMV检测和筛选的可行性。方法 ：采用PCR对119名献血员血清中HCMV-DNA进行检测，同时与ELISA法检测血清中HCMV-IgM、IgG的结果相比较。结果 ：献血员中HCMV-DNA、HCMV-IgM、IgG阳性率分别为9.2%(11/119)、4.2%(5/119)、92.4%(110/119)。同时显示HCMV-DNA阳性率随年龄增大而增高。结论 ：献血员中存在着HCMV感染，年龄较大者可能是HCMV传播的重要传染源。PCR法优于ELISA法，若条件许可，选用PCR法对献血员的血液进行HCMV检测和筛选是可取的。

    

     中国图书资料分类法分类号 R373

PCR DETECTION AND ANALYSIS OF HUMAN CYTOMEGALOVIRUS INFECTION IN BLOOD DONORS

Liang Hao

    (Department of Epidemiology,Guangxi Medical University,Nanning 530021)

Abstract Objective :To furtherreveal the state of human cytomegalovirus (HCMV) infection in blood donors,as well as toprobe into the possibility of detecting HCMV in blood and screening blood donors by meansof polymerase chain reaction (PCR).Methods :HCMV-DNA in serum samples of119 blood donors was detected by PCR.As a comparison,HCMV-IgM and HCMV-IgG weresimultaneously determined by using ELISA.Results :The positive rates ofHCMV-DNA ......