摘要： 为探讨中药化痰散结方诱导人肺癌细胞(SPC-A1)凋亡的作用及其对正常人胚肺细胞的毒性作用，通过血清药理学方法，选择不同浓度的含药兔血清与人肺癌细胞共同孵育处理，并以正常人胚肺细胞(HEL)作对照。结果：人肺癌细胞经含中药兔血清作用，由贴壁生长而逐渐脱落，外形变圆，体积缩小，折光性增强；荧光镜下可见细胞核破碎，裂解为大小不等的凋亡小体；流式细胞仪检测显现典型的细胞凋亡峰，以5%含药血清作用48h凋亡峰最为明显，凋亡率为42.8%；出现S期细胞阻滞；而该含药血清对人胚肺细胞无诱导凋亡作用。化痰散结方通过血清药理学方法诱导人肺癌细胞的凋亡，可能是其抗肿瘤的分子学机制之一。

ABSTRACT: Objective:Approach to the effect of composed Chinese drug 892 on inducing the apoptosis of SPC-A1.Methods: According to the basic TCM theory, the pathologic characteristic of lung canceris stagnation of phlegm and turbid qi. Drug 892, composed of Banxia (Rhizoma Pinelliae),Tiannanxing (Rhizoma Arisaematis), and Sanqi(Radix Notoginseng). After incubating SPC-A1and drug-bearing rabit serum which at different concentration with serologicpharmacological method, observed and detected the growth and apoptosis of cells, cellcycle and apoptosis rate. Results: By the action of drug-bearing rabit serum, SPC-A1 grewagainst the wall and then peeled off gradually; fluorescence microscope showed the nucleusbroke into pieces, and cell split into apoptosis bodies in different size; FCM showed atypical peak of apoptosis, which was most obvious after 48 hours' action with 5%drug-bearing rabit serum at the rate of 42.82%. Conclusion: One of the antineoplasticmolecular mechanism of composed Chinese drug 892 may be its effect on inducing theapoptosis of SPC-A1 by serologic pharmacological method.

     ......