关键词：肝炎病毒；基因型
摘要 目的：探讨本地区丙型肝炎病毒(HCV)感染者的基因分型。方法：应用逆转录聚合酶链反应(RT-PCR)进行HCV-RNA检测，并对其阳性产物进行HCV非结构5区的限制性片段长度多态性分析。结果：安徽省丙型肝炎患者HCV基因型有五种，即Ⅰ、Ⅱ、Ⅲ、Ⅳ、Ⅱ/Ⅲ型，其构成比分别为0.53%、75.66%、13.23%、0.53%、6.35%。丙肝患者不同病种与HCV基因型分布差异无显著性(P>0.05)，献血员与输血后丙型肝炎患者间分布差异无显著性(P>0.05)，血透与输血后丙型肝炎患者间HCV基因型分布差异有显著性(P<0.01)。结论：安徽省以HCV Ⅱ型为优势株，应加强对血透患者及献血员进行HCV筛检。
中国图书资料分类法分类号 R 373.2 Q 343.1
HCV genotypes in blood donors,haemodialysis and hepatitis patients
Wang Baolong,Su Hong,Ni Jinfa
(Anhui Provincial Clinic Laboratory Centre,Hefei 230001)
Abstract Objective :To study the distribution of HCV genotypes in Anhui province.Methods:Anti-HCV positive serum samples were collected from 371 hepatitis C and 60 haemodialysis patients and 100 blood donors,and detected with reverse transcription polymerase chain reaction(RT-PCR) for HCV-RNA.Based on the results of PCR positivity.HCV genotyping was investigated with restriction fragment length polymorphism.Results: The 5 genotypes detected among the patients were Ⅰ ,Ⅱ ,Ⅲ ,Ⅳ ,Ⅱ /Ⅲ and their proportion of occurrence were 0.53%,75.66%,13.23%,0.53%,6.35% respectively.No significant correlation could be detected between clinical progression of the liver disease and distribution of HCV genotypes(P> 0.05).There was also no significant difference in blood donors and post-transfusion hepatitis C(P> 0.05).But in haemodialysis patients and post-transfusion hepatitis C,there was significant difference in distribution of HCV genotypes(P< 0.01).Conclusions: HCV Ⅱ infection is the dominant genotype in Anhui province.It is essential to screen the HCV genotype of blood donors and haemodialysis patients.
......
您现在查看是摘要页，全文长 14205 字符。