马秀璟 袁成 李熔 马秀璟(中国人民解放军济南军区总医院 济南 250031)；李熔(河南省洛阳市商业职工医院 洛阳 471000) 解放军药学学报 1999 0 15 5


    关键词：高效液相色谱法；退烧冲剂；黄芩苷 期刊 jfjyxxb 0 研究简报 fur -->


    

摘 要 目的： 测定退烧冲剂中黄芩苷的含量。方法： 采用HPLC法，C₁₈ 柱，流动相醋酸缓冲液(pH4.5)-甲醇(1∶1)，检测波长254nm。结果： 黄芩苷在0.5～10mg^. L^-1 浓度范围内线性关系良好，r＝0.9998，平均回收率98.6%，RSD为1.59%。结论： 所建立的含量测定方法简便、准确，可作为该产品的质量控制方法。

    中图分类号 R927.2

DETERMINATION OF BAICALIN IN TUISHAO CHONGJI BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

Ma Xiujing，Yuan Cheng，Li Rong

    (General Hospital of Jinan Military Region,Ji’nan 250031)

Abstract AIM To establish a RP-HPLC method for determination of Baicalin in Tuishao Chongji.METHODS The contents of Baicalin was determined by HPLC,using Spherisorb C₁₈ (4×250mm) column,acetic acidbuffer(pH=4.5)-methanol(1∶1) as the mobile phase.The detective wavelength was 254nm.RESULTS The average recovery was 98.6% with the relative standard deviation (RSD) 1.59%(n=5).CONCLUSION This method is simple and accurate,Which can be used in the quality control of this product.

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