【摘要 】 目的 观察血管内液体球囊放射治疗对血管介入治疗后再狭窄的影响，为其临床应用提供实验依据；同时通过放射治疗效应来探讨放射治疗防止再狭窄发生的可能机制。方法 兔髂动脉经球囊过度扩张损伤后，一侧经³² P液体球囊血管内照射作治疗，另一侧未经治疗作对照。用计算机图像分析方法观察血管组织形态学的变化；免疫组化方法测定增殖细胞核抗原(PCNA )阳性细胞以了解血管内膜增殖过程。结果 兔髂动脉经球囊过度扩张损伤后，血管内膜可明显增生，PCNA染色为强阳性；经³² P液体球囊照射后的对侧损伤髂动脉，内膜增生明显受抑，PCNA 染色为弱阳性，外弹力板围绕面积均增加，管腔面积无明显变化。结论 ³² P液体球囊确可防止再狭窄的形成，其机制可能是通过抑制平滑肌细胞增殖和改善血管重塑形成。

Experimental study of endovascular brachytherapy using liquid ³² P-filled balloon catheter

Huo Yong, Wang Risheng, Chen Ming, et al. The First Hospital of Beijing Medical University, Beijing 100034

【Abstract 】 Objective To evaluate the effect of endovascular brachytherapy on restenosisafter interveneional therapy and investigate the possible mechanism of restenosisprevention after endovascular brachytherapy. Methods In balloon-injuried rabbit model, pathological sections ofiliac artery were observed to estimate the changes of vascular histomorphology by computeranalysis of photomicrogram and the proliferation of vascular smooth muscle cells wasevaluated by means of immunohistochemistry to quantify the proliferation of cellularnuclear antigen(PCNA).Results After rabbit iliac arteries were injuried by balloon overstretchangioplasty, PCNA staining showed less proliferative in the irradiation side than in thecontrol side. In the irradiation side, the fromation of neointima was suppressed, with EELarea increased but without lumen area change.Conclusion Endovascular brachytherapy using liquid ³² P-filled balloon catheter system could prevent restenosis,its mechanism might be the inhibition on the proliferation of neointima and improvement onvascular remodeling.

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