陈 功 黄 强 兰 青 周丽英 楼美清 沈 茜 王文仲 周晓平 215004 苏州医学院附二院脑外科(陈 功、黄 强、兰 青、周丽英)；上海，长海医院神经外科及免疫实验室(楼美清、沈 茜、王文仲、周晓平) 中华神经外科杂志 1998 0 14 3


    关键词：双特异抗体；IL-2；胶质瘤；LAK细胞 期刊 zhsjwkzz 0 论 著 fur -->


    

摘要 目的：探索提高胶质瘤治疗效果的新方法。方法：以化学偶联制备抗CD3-抗胶质瘤双特异性抗体，以³ H掺入法测定其与IL-2协同增强LAK细胞对胶质瘤的细胞毒性，以CD3单抗、胶质瘤单抗、二种单抗混和物及RPMI1640作对照。结果：双特异抗体与单抗相比，能显著提高LAK细胞对胶质瘤细胞的杀伤作用；双抗加入IL-2后，LAK细胞毒性得到显著的提高，IL-2也能增强抗CD3单抗和SZ39单抗的细胞毒性；同时发现，来源于恶性胶质瘤患者的LAK细胞毒性，在加入单抗、双抗和IL-2后，其细胞毒性与正常人相比，仍有显著性差异。结论：抗CD3-抗胶质瘤双特异抗体与IL-2协同可显著提高LAK细胞对胶质瘤的细胞毒作用。

Study on cooperativeaction of anti-CD3×anti-glioma bispecific antibody and IL-2 on cytotoxicity of LAK cells Chen Gong, Huang Qiang, Lan Qing,et al. Department of Neurosurgery Department, Second Affiliated Hospital, Suzhou MedicalCollege, Suzhou 215004

    Abstract Objective: To investigate a newmethod for improving the therapeutic effect on glioma. Method: The anti-CD3×antigliomabispecific antibody (BIAB) was built by chemical conjugating method. Cytotoxicity of LAKcells against glioma cells enhanced by the BIAB and IL-2 was examined with ³ H incorporation method, comparing with control groups: CD3antibody, anti-glioma antibody SZ39, mixture of the two antibodies, RPMI 1640. Results:Cytotoxicity of LAK cells against glioma cells could be markedly enhanced by BIAB comparedwith anti-CD3 and SZ39 monoclonal antibody (P< 0.01 or P< 0.05). When LAK cells incubating with IL-2, itscytotoxicity enhanced by BIAB was more high (P< 0.05). Also, we found that cytotoxicty of LAK cells frompatients with malignant glioma was lower than that from normal individuals when incubatingwith anti-CD3 and SZ39 monoclonal antibody, BIAB or IL-2(P< 0.05,or P< 0.01). Conclusion: Anti-CD3×anti glioma BIAB couldmarkedly enhance the cytotoxicity of LAK cells against glioma.

    Key words Bispecific antibody Interleukin-2 Glioma Lymphokine activated killer cells

自1986年Jacobs将LAK细胞用于人脑恶性胶质瘤的治疗以来 ......

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