赵志泉 张国新 徐顺福 倪黎 王学浩 210029 南京，南京医科大学第一医院 中华消化杂志 1999 0 19 2


    关键词：癌，肝细胞；基因表达 期刊 zhxhzz 0 论著 fur -->


    

【摘要】 目的 了解自分泌运动因子Autotaxin (ATX) mRNA在肝癌中的表达及其与肝癌临床病理特征之间的关系。方法 应用半定量逆转录聚合酶链反应(RT-PCR)研究5例正常肝组织、32例肝癌组织中Autotaxin mRNA的表达，以β-actin为内参照。结果 5例正常肝组织及32例肝癌中均有Autotaxin mRNA表达；但Autotaxin基因表达值(±s)癌组织显著高于正常肝组织(68.23%±15.31%对31.97±8.05%，P<0.001)。高表达ATX肝癌与癌细胞肝内转移、门静脉癌栓形成、肿瘤的分化程度有关，而与年龄、性别、病因、肿瘤大小等无关。结论 Autotaxin基因过度表达与肝细胞癌的进展、转移有关。

The expression of autotaxin mRNA in the human hepatocellular carcinoma

ZHAO Zhiquan, ZHANG Guoxin, XU Shunfu, et al.

    The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029

【 Abstract】 Objective To investigate the expression of Autotaxin (ATX) mRNA in the human hepatocellular carcinoma(HCC) and whether there is correlation between ATX expression and clinicopathological features of HCC.Methods Five Specimens of normal liver tissues and thirty-two histologically verified HCC specimens were studied. Semi-quantitative reverse transcription polymerase chain reaction was used to detect mRNA expression of ATX. Results ATX was expressed in all 32 HCC and 5 normal liver tissues. The expression level of ATX gene in HCC samples was higher than that in normal liver tissues (68.23%±15.31% vs. 31.97%±8.05%, P<0.001). Intrahepatic metastasis, vascular invasion and poor differentiation were more frequently noted in HCC patients with high ATX expression than in patients with low ATX expression.Conclusion The expression of ATX gene was related to HCC development and metastases.

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