王翔 张元芳 叶烈夫 丁强 瞿连喜 200040 上海医科大学泌尿外科研究所,华山医院泌尿外科 中华泌尿外科杂志 1999 0 20 5


    关键词：膀胱肿瘤,癌,端粒酶活性 期刊 zhmnwkzz 0 论 著 fur -->


    

【摘要 】 目的 检测尿脱落细胞端粒酶活性并探讨其临床意义。 方法 应用改良的端粒重复序列扩增(TRAP)-银染方法，分别对膀胱癌组织、正常膀胱组织，以及膀胱癌患者和非尿路上皮肿瘤患者的尿脱落细胞、膀胱冲洗液进行端粒酶活性检测。 结果 12例正常膀胱组织均无端粒酶活性，48例膀胱癌组织中44例(91.7%)端粒酶阳性。膀胱癌患者尿液及膀胱冲洗液中脱落细胞端粒酶阳性率分别为83.3%(40/48)和87.5%(42/48)。12例分化良好(G₁ 级)膀胱癌患者中，尿液和膀胱冲洗液中脱落细胞端粒酶阳性率分别为75.0%(9/12)和83.3%(10/12)。 结论 尿脱落细胞端粒酶活性检测敏感性高，可用于膀胱癌的早期诊断和术后随访。

Telomerase activity in exfoliated urothelial cells of bladder cancer WANG Xiang ,ZHANG Yuanfang ,YE Liefu ,et al .Department of Urology ,Huashan Hospital ,Institute of Urology ,Shanghai Medical University ,Shanghai 200040

【Abstract 】 Objective To study the telomerase activity in exfoliated urothelial cells of bladder cancer and to evaluate its clinical significance. Methods Telomerase activity was studied by means of a modified PCR-based telomeric repeat amplification (TRAP)-silver stain assay in tissue and urine samples and bladder washings from 48 cases of bladder cancer and 12 normal bladder and in urine samples and bladder washings from 20 patients with non-urothelial cancer. Results Telomerase activity was detected in 44 of the 48 tumor samples(91.7%)while none of the normal bladder tissue showed telomerase activity.Telomerase activity was also detected in 83.3%(40/48)of urine samples and 87.5%(42/48)of bladder washings from bladder cancer patients.In 12 patients with well diffentiated bladder cancer (G₁ ),telomerase activity was detected in 9 urine samples(75.0%)and in 10 bladder washings(83.3%). Conclusions Telomerase activity could be detected in most urine samples of bladder cancer patients and therefore would be a good diagnostic marker.But it is not related to the tumor grade or stage.

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