关键词:细胞学技术;细胞,培养的
【摘要】 目的 建立一种比较简便的人正常尿路上皮细胞无血清培养方法。 方法 标本组织块用冷消化法分离出尿路上皮细胞,培养于无血清生长培养基。观察传代细胞形态及生长情况,并用TGF-β1 抑制细胞增殖试验检验细胞在有血清或无血清培养基中对TGF-β1 的反应。 结果 培养的总成功率为73%,细胞具有典型的移行上皮细胞形态学特征,细胞能在24小时后增殖进入对数生长期,传代9~11次后开始衰退。另外,无血清培养体系比含血清培养体系更能反映出TGF-β1 对细胞抑制作用。 结论 此培养体系具有简单、短期内可扩增出大量纯净上皮细胞、应用范围广和培养成功率高的特点。
Culture of human normal urothelial cells with serum-free medium
TANG Yang,CHANG Jiwu,SUI Zhifang
et al.Department of Urology,The Second Hospital,Tianjin Urological Institute,Tianjin 300211
【Abstract】 Objective To establish a relative simple and convenient serum-free culture method of human unthelial cells. Methods Epithelial cells were isolated from sample tissue by cold trypsinization and cultured in serum-free growth medium.Growth and morphology of passaged cells were observed.Moreover,cells reacting to TGF-β 1 was cheked with TGF-β 1 inhibiting cell growth test in serum-free or containing serum medium. Results Total successful rate of culture was 73%.Cultured cells had typical morphological features of transitional epithelium and rapidly proliferated into logarithm growth period.After 9~ 11 passages,cells began senescence.The changes of cell proliferation in serum-free medium rather than serum containing medium reflected the TGF-β 1 effect. Conclusions This culture system was simple and convenient,amplifying a lot of pure epithelial cells in short term with more applicability and high successful culture rate.
......
您现在查看是摘要页,全文长 10229 字符。