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关键词：肾肿瘤；癌；端粒酶
【摘要】 目的 探讨端粒酶活性与肾细胞癌之间的关系。 方法 采用改良TRAP法对32例肾细胞癌组织及正常肾组织和可疑癌旁组织中端粒酶活性进行检测,同时了解其与临床生物学特征的关系。 结果 32例肾细胞癌组织中端粒酶强阳性17例,阳性12例,阴性3例,阳性率91%;32例正常肾组织中端粒酶弱阳性者仅2例,阳性率6%;可疑癌旁组织阳性者6例,阳性率19%。三组端粒酶活性比较,阳性率差异有显著性(P<0.01)。 结论 肾细胞癌组织中端粒酶活性检出率高,特异性强,无假阳性和假阴性。提示端粒酶活性检测可作为判断肾肿瘤恶性程度的标记物，并可能成为判断肾肿瘤预后的一项指标。
Telomerase activity in renal cell carcinoma
JIA Ruipeng,CHENG Jiyi,MA Qingzheng
et al.Department of Urology,Shandong Provincial Hospital,Jinan 250021
【Abstract】 Objective To study the telomerase activity in human renal cell carcinoma and to evaluate its correlation with the clinicobiologic features of the neogrowth. Methods The telomerase activity was studied by means of a polymerase chain reaction based telomerase activity assay (TRAP) in 32 renal cell carcinoma tissues,32 normal renal tissues and 32 paracancer tissues and its correlation with the clinicopathologic features of the tumor was evaluated. Results Telomerase activity was strongly positive in 17 renal cell carcinoma tissues,positive in 12 and negative in 3,the total positive rate being 91%.In 32 samples of normal renal cortex tissues only 2 was weakly positive (6%) and it was positive in 6 paracancer tissues (19%).The difference was conspicuous (P< 0.01). Conclusions The positive rate of telomerase activity was significantly higher in renal cell carcinoma tissues and might serve as a prognostic marker for estimating the biologic characteristics of renal cell carcinoma.
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