涂响安 赵志毅 黄春南 余明年 谢佛龙 冯家骅 何炳辉 刘丽萍 李全贞 左连富 涂响安 赵志毅 黄春南 余明年 谢佛龙 冯家骅 何炳辉 刘丽萍 526021 广东 肇庆市第一人民医院泌尿外科;李全贞 中山医科大学达安基因诊断中心;左连富 河北省肿瘤研究所 中华泌尿外科杂志 1999 0 20 10


    关键词：泌尿系肿瘤；基因；预后 期刊 zhmnwkzz 0 论著 fur -->


    

摘要 目的 研究ras、p53基因突变和蛋白表达及DNA倍体异常与尿路上皮肿瘤预后关系。 方法 应用PCR-SSCP和流式免疫荧光法检测52例尿路上皮肿瘤K-ras和p53基因突变及蛋白表达和DNA含量变化。 结果 K-ras、p53基因突变分别为5例(9.6%)和22例(42.3%)；ras p21、p53蛋白阳性表达分别为36例(69.2%)和33例(63.5%)；异倍体和二倍体肿瘤分别为22例(42.3%)和30例(57.7%)；DNA倍体异常、p53基因突变及ras p21和p53蛋白表达与肿瘤病理分级、分期及预后密切相关(P<0.01)；异倍体肿瘤其p53基因突变、ras p21和p53蛋白表达率均明显高于二倍体肿瘤(P<0.01)。 结论 DNA倍体异常、p53基因突变及ras p21和p53蛋白表达对尿路上皮肿瘤预后判断有重要意义。

ras、 p53 gene mutation and ras p21、 p53 protein overexpression and DNA abnormality in urothelial tumors

TU Xiangan, ZHAO Zhiyi,HUANG Chunnan,et al.

    Department of Urology, the First People's Hospital,Zhaoqing City, Guangdong 526021

Abstract Objective To study K-ras、p53 gene mutation,ras p21 and p53 protein overexpression and DNA ploidy abnormality in urothelial tumors. Methods In 52 cases of urothelial tumors,K-ras and p53 gene mutations were studied by means of polymerase chain reaction and single-stranded conformation polymorphism.ras p21 and p53 overexpression and DNA content were studied by flow cytometry and cellular immunofluorescence technique. Results K-ras mutation was observed in 9.6% of the cases and p53 mutation in 42.3%.The positive protein expression rates of ras p21 and p53 were 69.2% and 63.5% respectively.22 (42.3%) were aneuploidy tumors and 30 (57.7%) diploidy tumors.DNA ploidy abnormality,p53 gene mutation and the positive expression of ras p21 and p53 proteins increased well with the rise of tumor grade and stage denoting a bad prognosis (P<0.01).In aneuploidy tumors,p53 gene mutation and ras p21,p53 protein expression were higher than those in diploidy tumors (P<0.01). Conclusions DNA ploidy abnormality,p53 gene mutation and ras p21,p53 protein overexpression might serve as markers for the prognosis of urothelial tumors.

    Key words Urologic neoplasms Genes Prognosis

我们应用聚合酶链式反应加单链多态性分析(PCR-SSCP)及流式细胞术和细胞免疫荧光技术检测52例尿路上皮肿瘤凋亡基因ras和p53突变及ras和p53蛋白表达和DNA倍体变化 ......