摘要 为适应临床器官移植组织配型的要求，采用酚氯仿快速DNA提取技术，建立起2.5小时完成的PCR-SSP-HLA-DRB基因分型方法，并与血清学实验进行对照实验。结果显示，850份标本DNA提取数量平均为14.9μg，DNA平均纯度为1.6；静脉血和腹腔血之间的HLA-DRB1抗原频率无显著性差异，DNA质量有显著性差异。结果表明此法具有快速、准确和适应腹腔血HLA-DNA分型的优点。

    Rapid PCR-SSP-HLA-DRB DNA based typing Chen Hongtao, Xiao Lulu, Yu Lixing, et al. Guangzhou Tissue TypingCenter, Guangzhou 510095

    Abstract PCR-SSP technique for HLA-DRB typing, which takes 2.5 hours, hasbeen established with rapid mini scale chloroform out DNA extraction technique. The resultindicates that the mean weight of extracted DNA is 14.9μg per 500μl blood and the meanratio of A260/A280 is 1.6. No significant deviation in antigen frequency distribution wasfound between venous blood and abdominal cavity blood but there has been significantdeviation of extracted DNA between the two. PCR-SSP technique was claimed to be rapid,accurate and suitable for DNA based typing of abdominal cavity blood.

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