李新武 孙守红 李涛 张流波 刘秀岩 袁洽劻 100021 北京，中国预防医学科学院消毒检测中心 中华流行病学杂志 1999 0 0 6


    关键词：酸化电位水；杀菌因子；有效氯；蛋白胨 期刊 zhlxbxzz 0 论著 fur -->


    

【摘要】 目的 比较不同厂家生产的酸化电位水生成机制备的酸化电位水对微生物的杀灭效果，并对杀菌因子和影响因素进行探讨。方法 应用悬液定量杀灭试验检测酸化电位水对金黄色葡萄球菌、大肠杆菌和枯草杆菌黑色变种芽胞的杀灭效果，HCl+次氯酸钠和蒸馏水+次氯酸钠对金黄色葡萄球菌的杀灭效果及有机物对酸化电位水杀灭枯草杆菌黑色变种芽胞效果的影响。应用HBsAg抗原性悬液定性破坏试验检测酸化电位水对HBsAg抗原性的破坏效果。结果 所试厂家的生成机制备的酸化电位水对金黄色葡萄球菌、大肠杆菌作用30s其平均杀灭率均为100%, 对 HBsAg 作用30s，可破坏HBsAg的抗原性。蒸馏水中加入次氯酸钠，其杀菌效果与酸化电位水相同。在枯草杆菌黑色变种芽胞悬液中加与不加1%的蛋白胨， 酸化电位水对该芽胞作用20min，其平均杀灭率分别为59.54%和100%。结论 所试厂家的生成机制备的酸化电位水对金黄色葡萄球菌、大肠杆菌和HBsAg具有快速的杀灭和破坏作用。酸化电位水的主要杀菌因子是有效氯。1%蛋白胨对酸化电位水杀灭芽胞的效果有明显影响。

    

Study on germicidalefficacy of the electrolyzed oxidizing water from the instruments produced by differentmanufacture

LI Xinwu, SUN Shouhong, LI Tao,etal.

Centre of Disinfection Detection,Chinese Academy of Preventive Medicine, Beijing 100021

【 Abstract】 Objective To compare the germicidal efficacy of ElectrolyzedOxidizing Water (EOW) from instruments offered by the manufactures.To approachbactericidal factors of EOW and factors influencing the germicidal efficacy. Methods A quantitativesuspension test was used for detecting the germicidal efficacy of EOW against Staphy aureus, Escherichia coli and spores of Bacillus subtilis var.niger. This test was alsoused for detecting the germicidal efficacy of HCl+NaOCl against Starphy aureus andobserving the influence of 1% peptone on efficacy of EOW in killing spores of Bacillussubtilis var.niger. Results It was found that EOW produced by every instrument of themanufactures with 30 seconds contact-time could attain a killing rate of 100% for Starphyaureus and E.coli and could destroy the antigenicity of HBsAg. The germicidal efficacy ofthe NaOCl dissolved in distilled water against Starphy aureus was the same as of EOW. Asthe suspension of spores of Bacillus subtilis var. niger containing or no containing 1%peptone, EOW with 20 minutes contact time could attain a killing rate of 59.94% or 100%respectively. Conclusion EOW produced by instruments of all the manufactures can rapidlykill or destory the Starphy aureus, Escheichia coli, the antigenicity HBsAg. The maingermicidal factor of EOW is available chlorine. The 1% peptone has significant influenceon germicidal efficacy of EOW.

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