摘要 目的 观察腺病毒载体介导的人野生型Rb基因导入后促进血管平滑肌细胞衰老的作用，并探讨Rb基因抑制平滑肌细胞增生的机理。 方法 用外源性Rb基因重组腺病毒载体感染体外培养的兔主动脉血管平滑肌细胞。以细胞超微结构、³ H-胸腺嘧啶核苷掺入、流式细胞术分析细胞周期和β-半乳糖苷酶染色等方法观察平滑肌细胞的衰老变化。 结果 Rb基因导入可使平滑肌细胞发生衰老，表现为细胞线粒体肿胀，脂褐素堆积，DNA合成减少，细胞停滞在G₀ /G₁ 期，与衰老相关的β-半乳糖苷酶表达增加。

结论 野生型Rb基因通过促进细胞衰老而对平滑肌细胞增生起抑制作用。

Abstract Objective To observethe promotion of the senescence of vascular smooth muscle cells (SMC) after theintroduction of retinoblastoma (Rb) gene via a recombinant adenovirus vector and toexplore the mechanism of inhibition of SMC proliferation by wild-type Rb gene. Methods Areplication-deficient adenovirus vector encoding a wild-type Rb was constructed to infectthe cultured rabbit aortic SMC. The senescent changes of SMC were observed withtransmission electron microscope, ³ H-thymidineincorporation test, β-galactosidase stain and cellcycle analysis with flow cytometry. Results Introduction of wild-type Rb gene into SMC could arrest thecells at G₀ /G₁ phase of the cell cycle and lead to inhibition of DNAsynthesis.The senescent SMC showed swelling of mitochondria, accumulation of lipofuscinand positive senescence-associated β-galactosidasestain. Conclusions The results demonstrate that the wild-type Rb gene suppresses SMCproliferation by promotion of cell senescence.

     ......