杨卫平 姜泗长 杨伟炎 苏振伦 郭维 100853 北京市，解放军总医院耳鼻咽喉研究所 中华老年医学杂志 1998 12 17 6


    关键词：耳蜗；钾通道；老年性聋 期刊 zhlnyxzz 0 基础研究 fur -->


    

摘要 目的 观察爪蟾卵母细胞表达的老年大鼠耳蜗核电压依赖性离子通道电流的变化，为研究感音神经性聋听觉中枢离子通道的变化建立方法学基础。 方法 分别自青年和老年大鼠耳蜗核提取多聚腺嘌呤信使核糖核酸(mRNA)，注入非洲爪蟾卵母细胞表达有功能的钾离子通道，并利用电压钳方法记录电压依赖性的离子通道电流。 结果 在注射老年大鼠耳蜗核mRNA的卵母细胞膜上记录到电压依赖性的钾离子通道电流最大幅度(278±37nA,16只)，明显小于青年组(364±42nA，17只，P<0.001)。 结论 移植的老年大鼠耳蜗核钾离子通道功能的下降可能与老年性聋的发病有关。

Potassium channel expression in Xenopus oocytes by messenger RNA from aged rat cochlear nucleus

Yang Weiping, Jiang Sichang, Yang Weiyan, et al. Institute of Otolaryngology, General Hospital of PLA, Beijing 100853

Abstract Objective To detect the change of voltage-dependent on channels expressed in Xenopus oocytes by mRNA from aged rat cochlear nucleus so as to establish a methodological foundation for studying the changes of ion channels in auditory centre with sensorineural hearing loss. Methods Poly (A)⁺ mRNAs isolated from the cochlear nucleus of the adolescent(2-3 months) and aged (20-36 months) rats by acid guanidinum thiocyanate-phenol-chloroform (AGPC) method and Promega's new magesphere technique were microinjected into Xenopus laevis oocytes to express functional ion channels. Voltage clamp technique was used to record the current of voltage-dependent ion channels. Results Voltage-dependent potassium channel was detected from oocytes injected with adolescent and aged rat cochlear nucleus mRNA. The maximum amplitude of potassium current detected in aged group was lower than that in adolescent group (P<0.001). Conclusions The result indicates that the decrease of potassium channel function in aged rat cochlear nucleus may be related to presbycusis.

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