雌激素对卵巢切除雌兔血管内膜损伤后血小板聚集与血管平滑肌增殖的影响
雌激素类|血管内膜|血小板聚集,关键词：
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关键词：雌激素类；血管内膜；血小板聚集
摘要 目的 探讨不同剂量雌激素对切除卵巢的雌兔血管内膜损伤后血小板聚集与血管平滑肌增殖的影响。 方法 切除卵巢的成年雌性大白兔50只，行主动脉球囊扩张术，造成血管内膜损伤实验模型，采用氚标记胸腺嘧啶脱氧核苷( 3H-TdR)掺入测定雌激素对血管平滑肌细胞(VSMC)增生的影响，采用图像分析仪测定新生内膜增生面积及Born比浊法测定雌激素对血小板聚集的影响。 结果 单纯切除卵巢的雌兔，未给予苯甲酸雌二醇(E2 )者，其血小板聚集率显著高于其它各组(P<0.01)，血管平滑肌细胞增生程度虽有增加，但与对照组比较差异无显著性(P>0.05)；每天肌肉注射E2 2μg/kg不能抑制血小板聚集与血管平滑肌增殖；而每天20μg/kg即可显现明显的抑制平滑肌增生作用，其血小板聚集率明显下降；但每天20及100μg/kg其抑制作用差异无显著性(P>0.05)。 结论 一定剂量的雌激素能有效的抑制血小板聚集和血管平滑肌增殖。
Effects of estrogen on platelet aggregation and vascular smooth muscle cell proliferation in injured arterial intima of ovariectomized female rabbits
Feng Li, Zhang Yuanhui, Wu Saizhu, et al. Nanfang Hospital, the First Military Medical University, Guangzhou 510515
Abstract Objective In order to provide new experimental evidence for the prevention and treatment of restenosis after percutaneous transluminal coronary angioplasty or coronary artery disease in postmenopausal women, we investigated the effect of different doses of estrogen on platelet aggregation and vascular smooth muscle cell (VSMC)proliferation of ovariectomized female rabbits with abdominal aortic arteries undergone balloon injury. Methods Abdominal aortic arteries of fifty ovariectomized New Zealand female rabbits were undergone balloon injury. By uptaking 3 H-TdR and Born turbidity method, the effects of estrogen on VSMC proliferation and platelet aggregation were observed and the intimal thickening areas were measured by image pattern analysis method. Results In ovariectomized rabbits without estradiol therapy the platelet aggregation increased significantly in comparison with other groups (P< 0.01). Although VSMC proliferation had increasing tendency, there was no statistical significance comparing with control group (P> 0.05). The platelet aggregation and VSMC proliferation were not inhibited by treatment with benzoate estradiol 2μ g*kg-1 *d-1 ,im. But treatment with benzoate estradiol 20μ g*kg-1 *d-1 ,im showed significant inhibition. There was no significant difference between treatment with 20 and 100 μ g*kg-1 *d-1 ,im (P> 0.05). Conclusions Benzoate estradiol over 20μ g*kg-1 *d-1 can inhibit VSMC proliferation and platelet aggregation effectively.
Key Words Estrogen Tunica intimal Platelet aggregation
补充雌激素治疗可明显降低绝经期妇女冠心病的发病率及与死亡率〔1〕 ......
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