【摘要】 目的 探讨在促癌剂协同下Epstein-Barr病毒(EBV)对人胚鼻咽上皮的感染和转化能力。方法 体外培养的人胚鼻咽上皮分别接受EBV、EBV+佛波酯(TPA)或TPA处理，不同时间测量细胞生长晕大小，收集细胞，涂片，HE染色作形态测量；移植了人胚鼻咽组织的裸小鼠分别接受多次EBV、EBV+TPA皮下注射，处死动物后对移植物作常规病理切片观察。结果 体外培养的人胚鼻咽上皮在EBV转染后(单用EBV或在TPA协同下)，生长能力增加，核浆比增大；在体内，EBV感染(单用EBV或在TPA协同下)可引起移植于裸小鼠的人胚鼻咽组织癌变(原位癌与早期浸润癌各1例)。在体外，单独使用TPA没有诱癌作用，但似能增加EBV的感染率，尤其在早期；在体内，在TPA协同下，EBV能诱发早期浸润癌。结论 提示EBV在体外能使人胚鼻咽上皮细胞出现恶性转化倾向，及引起移植于裸小鼠的人胚鼻咽组织癌变，TPA能促进EBV感染和致癌作用。

Abstract In order to detect the ability of EBV on transfecting and transforming human nasopharyngeal epithelia(HNE) isolated from human embryo, the HNE was cultured in vitro and then transplanted into nude mice. HNEs were treated by EBV, EBV+TPA and TPA in vitro, respectively. The mice transplanted with HNE tissues were injected subdermally by EBV, EBV+TPA and distilled water several times, respectively. The morphological changes indicated that EBV induced not only enhancement of foci and N/C ratio in cultured HNE which are important characteristics of malignant transformation, but also carcinogenesis of transplanted HNE, all of the carcinoma were EBV positive. TPA seems to increase EBV transfection rate (EBV PCR positive rate) in vitro, especially in the early stage. TPA alone did not exert effect on malignant transformation. Cooperating with TPA, EBV induced early invasive carcinoma in vivo.

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