刘杞 王志毅 罗娅 黄爱龙 张定凤 刘杞 王志毅 罗娅 黄爱龙 张定凤 重庆医科大学病毒性肝炎研究所 中华肝脏病杂志 1999 0 7 3


    关键词：人肝再生增强因子 阅读框 cDNA克隆 期刊 zhgzbzz 0 实验研究 fur -->


    

摘要 目的 获取人肝再生增强因子(ALR)阅读框的cDNA克隆，为进一步研究打下基础。 方法 从人胎肝中提取总RNA作为模板，以寡聚dT为引物逆转录合成第一链cDNA，用我们设计的引物和PCR方法扩增双链cDNA。PCR产物约380bp并被亚克隆入pUC19，经测序和PCGENE软件分析。 结果 获得人ALR完整阅读框的cDNA片段，长度为378bp。与大鼠ALR和最近报道的人ALR序列比较同源性分别为86.5%和99.2%。 结论 成功地克隆人ALR完整阅读框的cDNA，同时提示人ALR参与了人胎儿晚期发育过程中胎肝的生长调节。

    

The cDNA clone and sequence analysis of the coding region of human augmenter of liver regeneration (hALR) gene

LIU Qi, WANG Zhiyi, LUO Ya, et al.

    Institute of Viral Hepatitis, Chongqing University of Medical Sciences, Chongqing 400010

Abstract Objective To obtaining cDNA clone of the coding region of human augmenter of liver regeneration(hALR) as a basis for further study. Methods Total RNA of human fetal liver was extracted as a template. First strand of cDNA was synthesised by reverse transcription with olig(dT)-primer and then amplifyed by PCR with the primers designed by ourself. The size of PCR product was about 380bp. it was subcloned into pUC19 and was sequenced by auto-sequencing instrument. The sequence of hALR was analysed with PCGENE program. Result The cDNA of integrity coding region of hALR was obtained. Its size was 378bp. The sequence homology of our hALR compared with rat ALR and human ALR recently reported were 86.5% and 99.2%, respectively. Conclusion It was also sugested that hALR takes part in growth regulation of fetal liver during human fetus late development.

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