杨冬华 张鸣青 杜江 徐重 粱巧明 毛积芳 覃汉荣 范子荣 510282，广州，第一军医大学珠江医院消化科(杨东华，张鸣表，覃汉荣，范子荣); 分子生物学实验室(杜江，徐重，王巧明); 上海第二军医大学生物化学研究室(毛积芳) 中华肝脏病杂志 1999 0 7 1


    关键词：癌，肝细胞 胰岛素样生长因子Ⅱ 基因，抑制，肿瘤 核糖核酸，反义 期刊 zhgzbzz 0 基础研究 fur -->


    

摘要 目的 研究人胰岛素样生长因子Ⅱ(IGF-Ⅱ)反义RNA对肝癌细胞株SMMC-7721的抑制效应。方法 人IGF-Ⅱ cDNA 0.1kb反向插入真核细胞表达载体pcDNA3，获得IGF-Ⅱ反义RNA表达载体pIGF-ⅡAs,将其导入人肝癌细胞株SMMC-7721,观察软琼脂培养集落形成的能力。流式细胞仪(FCM)测定pIGF-ⅡAs表达IGF-Ⅱ反义RNA对SMMC-7721细胞生长的影响。结果 转导入pIGF-ⅡAs的SMMC-7721细胞，不能在软琼脂上形成集落，FCM证实导入pIGF-ⅡAs细胞S期增多，而对照组SMMC-7721细胞和带pcDNA3空载体的SMMC-7721细胞则无明显变化。结论 pIGF-ⅡAs反义RNA体外可抑制肝癌细胞株SMMC-7721的致瘤性。

    

Inhibitory effect of IGF-Ⅱantisense RNA on malignant phenotype of hepatocellular carcinoma

YANG Donghua,ZHANG Mingqing,DU Jiang,et al.

Department of Gastroenterology,ZhujiangHospital,The First Military Medical University,Guangzhou 510282

Abstract Objective Inhibitory effect of insulin like growth factor Ⅱ(IGF-Ⅱ) antisense RNA on malignantphenotype of hepatic cancer cells was studied.Methods A 0.1kb cDNA ofhuman IGF-Ⅱ was reversely inserted into a eukaryotic expression vector of pcDNA3 and an IGF-Ⅱ antisense RNA expression vector of pIGF-Ⅱ As was obtained.The pIGF-Ⅱ As wasthen introduced into human hepatic cancer cell line SMMC-7721.The effect of IGF-Ⅱantisense RNA which was expressed by pIGF-ⅡAs on SMMC-7721 cell cycles progression wasmeasured with FCM.Results The coloning formation in anchorage-independentassay of SMMC-7721 cells with pIGF-ⅡAs was significantly decreased by comparision withcontrol groups of SMMC-7721 cells and SMMC-7721 cells transfected with pcDNA3 vectoralone.FCM analysis showed that the S phase of cell cycles for SMMC-7721 cells with pIGF-ⅡAswas increased,but there were no obvious changes in the control groups of SMMC-7721 cellsand SMMC-7721 cells with pcDNA3.Conclusion The pIGF-ⅡAs of IGF-Ⅱantisense RNA acts as an inhibitor on carcinogenesis of the SMMC-7721 cells in vitro.

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