郑起 汤钊猷 薛琼 200032 上海医科大学中山医院、肝癌研究所(郑起现在上海市第六人民医院普外科) 中华肝脏病杂志 1998 0 0 3


    关键词：肝素 肝细胞癌 肿瘤侵袭 纤溶酶原激活物 期刊 zhgzbzz 0 肝癌专栏 fur -->


    

【摘要】 目的 观察肝素对肝细胞癌(HCC)生物学特性的影响。方法 构建裸鼠肝癌转移模型40例，分肝素、抑肽酶和止血芳酸干预治疗组、生理盐水对照组，比较肿瘤生长、转移和宿主生存。用免疫组化方法和酶活性检测方法，检测实验各组血浆和肿瘤组织中尿激酶型纤溶酶原激活物(uPA)和纤溶酶原激活物抑制剂(PAI-1)的变化。结果 干预治疗30天肝素治疗组与对照组比较，肿瘤直径为1.50±0.61cm比2.98±0.50cm(P<0.001)；肿瘤腹壁浸润率为20%比100%(P<0.05)；肺转移率为20%比60%；50天生存率为60%比0。其他治疗组与对照组比较结果差异无显著性。免疫组化和PAI-1活性检测结果表明：肝素治疗组肿瘤组织中uPA和PAI-1阳性率低于对照组，PAI-1活性有降低趋势。结论 在肝癌转移模型中，肝素有抑制肿瘤生长、抑制肿瘤浸润转移和提高宿主生存期的作用。

    

ROLE OF HEPARIN ON TUMORGROWTH AND METASTASIS IN MODEL OF HUMAN HEPATOCELLULAR CARCINOMA IN NUDE MICE

Zheng Qi, Tang Zhaoyou, Xue Qiong. Liver Cancer Institute, Zhongshan Hospital, Shanghai Medical University, Shanghai200032

【Abstract】 Objective To observe the role of heparin in biologicalbehaviour of hepatocellular carcinoma (HCC). Methods 40 nude mice withLCI-D20 metastatic model of human HCC (MMHCC) were established. Heparin, aprotinin andaminomethylbenzoic acid were used for test to intervene HCC progression in MMHCC, and tocompare with the saline control. The changes of plasminogen activator (uPA) and itsinhibitor (PAI-1) were determined by PAI-1 activity kit and immunohistochemistry withmonoclonal antibody against uPA and PAI-1. Results When theheparin-treated group was compared to the control group after treating for 30 days, tumorsize was 1.50± 0.61cm vs 2.98±0.50cm(P<0.001), tumor local invasion rate was20% vs 100% (P<0.05), lung metastasis rate was 20% vs 60% and 50 days survival rate was60% vs 0 respectively. Conclusion Our investigation suggested thatheparin could not only inhibit tumor growth, but also inhibit tumor invasion andmetastasis, and improve host survival in MMHCC.

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