郭亚兵 冯筱榕 戴炜 侯金林 卢桥生 梁炽森 骆抗先 Peter Karayiannis 510515,广州,第一军医大学南方医院传染科(郭亚兵,冯筱榕,侯金林,卢桥生,骆抗生)；深圳东湖医院(戴炜) 中华肝脏病杂志 1999 0 7 1


    关键词：乙型肝炎病毒 启动子 变异 氯霉素乙酰转移酶 期刊 zhgzbzz 0 临床研究 fur -->


    

摘要 目的 乙型肝炎病毒(HBV)C基因调节序列存在较多变异，为探讨在诸多变异中，T₁₇₆₂ A₁₇₆₄ 变异对C区启动子(CP)活性究竟起何作用。方法 用克隆及PCR产物直接测序，检测T₁₇₆₂ A₁₇₆₄ 变异；并将含调节序列的PCR产物插入到氯霉素乙酰转移酶(CAT)表达质粒中，转染HepG₂ 细胞并瞬时表达。结果 通过比较野生株及变异株在CP区序列及CAT表达水平显示，T₁₇₆₂ A₁₇₆₄ 变异在使CAT表达下调中起主要作用。结论 T₁₇₆₂ A₁₇₆₄ 变异在使CP活性下调中起主要作用。

    

Effect of mutation (T1762A1764) onhepatitis B virus core promoter activity

GUO Yabing,FENG Xiaorong,DAI Wei,etal.Department of Infectious Disease,Nanfang Hospital,Guangzhou 510515

Abstract Objective With the knowledge that the substituted mutation of T₁₇₆₂ A₁₇₆₄ inhepatitis B virus (HBV) core promoter (CP) region was the most common variation duringchronic HBV infection,we proceed to study the role that the mutation T₁₇₆₂ A₁₇₆₄ plays in effecting the core promoter.Methods Seven patients withfulminant hepatitis and one asymptomatic HBV carrier were investigated for screening T₁₇₆₂ A₁₇₆₄ mutation by cloning and PCR products direct sequencing.PCR products containing HBV precore/core gene regulatory sequences were directly cloned into chloramphenicolacetyltransferase (CAT) expressing plasmid.CAT assay was done to compare CAT expressinglevel after transfecting into HepG₂ cell line and transient expression.Results Results reveal that T₁₇₆₂ A₁₇₆₄ mutation was the main factor thatrepresses CAT expression in vitro.Conclusion Among the several othervariations within hepatitis B virus' core promoter region,T₁₇₆₂ A₁₇₆₄ mutation has mainly affected the down-regulating activity of core promoter.

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