王爱民 杨跃伟 张斌 张继友 梁保忠 孙志军 710038 西安，第四军区大学唐都医院传染科 中华肝脏病杂志 1998 0 0 2


    关键词：实验性肝纤维化 Ⅳ型胶原酶 活性 期刊 zhgzbzz 0 实验研究 fur -->


    

【摘要】 目的 了解实验性肝纤维化过程中Ⅳ型胶原降解酶活性的动态变化。 方法 以人血白蛋白免疫损伤性大鼠肝纤维化为模型；检测实验性肝纤维化各阶段肝组织及血清Ⅳ型胶原降解酶活性的变化。 结果 肝组织活化及潜在Ⅳ型胶原降解酶活性在致敏阶段无明显变化；在尾静脉攻击中期显著升高；在尾静脉攻击后较前明显下降；在造模后1.5月及3个月时，潜在Ⅳ型胶原酶活性仍显著高正常对照组。血清Ⅳ型胶原降解酶活性与肝组织潜在Ⅳ型胶原降解酶活性呈显著正相关。 结论 血清Ⅳ型胶原降酶活性的变化反映了肝组织Ⅳ型胶原降解酶活性的改变。

    

TYPE Ⅳ COLLAGENASES ACTIVITY IN THE PROCESSES OF EXPERIMENTAL LIVERFIBROSIS

Wang Aimin, Yang Yaowei, ZhangBin, et al. Central Laboratory, 252 Hospital of PLA, Baoding, 071000

【Abstract】 Objective Toinvestigate the alteration of type Ⅳ collage-degradingenzyme activity in the processes of experimental liver fibrosis. Methods Immunecomplex induced animal model of liver fibrosis was developed in Wistar rats. Active andlatent hepatic type Ⅳ collagen-degrading enzymeactivity was measured. Results Active and latent hepatic type Ⅳ collagen-degrading enzyme activity was not altered significantlyduring immunizing period and were increased remarkably in the middle of intravenousbooster period. Then, they declined gradually. But three months after the modelproduction, latent hepatic type Ⅳ collagen-degradingenzyme activity was still much higher than that of normal control groups. Serum type Ⅳ collagen-degrading enzyme activity was correlatedsignificantly with hepatic type Ⅳ collagen-degradingenzyme activity. Conclusion Hepatic type Ⅳ collagen-degradingenzyme activity rose significantly in the processes of fibrogenesis, and decreased in thestage of liver cirrhosis and maintained at relatively high levels during reversal of livercirrhosis. Alterations of serum type Ⅳ collagen-degradingenzyme activity could reflect the changes of hepatic type Ⅳ collagen-degrading enzyme activity.

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