李晓林 余楠生 杜靖远 刘礼初 饶振玉 朱通伯 510120广州医学院第一附属医院骨科(李晓林、余楠生);武汉，同济医科大学协和医院骨科(杜靖远、刘礼初、饶振玉、朱通伯) 中华骨科杂志 1999 0 19 5


    关键词：碎石术骨痂骨折愈合 期刊 zhgkzz 0 基础研究 fur -->


    

【摘要 】目的观察高能震波(HESW)对骨痂中骨形成蛋白(BMP)表达的影响,探讨其促进骨折愈合的机理。方法手术造成家兔双侧胫骨中段标准骨折模型,对一侧胫骨骨折部施以HESW，另一侧为对照组。术后每周进行X线及组织学检查,并取骨痂组织切片用BMP抗体进行免疫组化染色(SABC法)。结果实验组骨痂中BMP表达强度在第2～4周时明显大于对照组(P<0.01),且X线摄片及组织学观察均显示实验组骨折愈合时间早于对照组.结论 HESW能够增加骨痂中BMP的表达,加强诱导成骨,从而促进骨折愈合.其可望成为一种非手术治疗骨折愈合障碍的新方法.

    The Influence of High Energy Shock Waves on Expression of Bone

    Morphogenetic Protein in the Fracture Callus in Rabbits

LI Xiaolin^* ,YU Nansheng，DU Jingyuan,et al.

    ^* Department of Orthopaedic Surgery,The First Affiliated Hospital,Guangzhou Medical College,Guangzhou 510120

【Abstract 】 Objective To detect the effects of high energy shock waves(HESW) on fracture healing and to explore its mechanisms.Methods Standardized fracture models were made in bilateral tibial diaphyses in 30 rabbits. Seven days after fracture, one side of the model was treated with HESW(14.5 kV,500 shock waves),the another side served as controls.The X-ray films and histological sections were made for the evaluation of effects on fracture healing.At the different stages,rabbits fracture callus and BMP antibody were adopted for immunohistochemical staining SABC method).Results The fracture healing found in experimental group were earlier than that of the control group.The intensity of the positive staining of experimental group was more than that of the control group (P<0.01)Condlusion The result suggests that HESW can increase the expression of BMP in fracture callus,and can stimulate callus formation and accelerate fracture healing.

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