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关键词：乙型肝炎病毒 树 动物模型
树实验感染乙型肝炎病毒的再评价
【摘要】 目的 评价乙肝病毒(HBV)实验感染树的稳定性与实用性。 方法 用含HBV的2.2.15细胞或乙肝患者混合血清以不同剂量、经不同途径接种60只健康树。观察其临床表现，检测血清ALT、HBsAg、HBeAg及HBV-DNA。肝组织行HE及免疫组织化学染色。 结果 部分树出现一过性食欲不振、稀便； 60只树不同时相ALT水平均在正常范围；8只树HBV标志物一过性阳性；肝组织未见类似于人乙型肝炎的典型病理改变；免疫组织化学染色为阴性。 结论 树不能持续稳定感染HBV。
EVALUATION OF EXPERIMENTAL INFECTION BY HUMAN HEPATITIS B VIRUS IN ADULT TREE SHREWS
Wang Haiping, Yao Zhiqiang, Zhou Yongxing, et al. Department of Infectious Diseases, Tangdu Hospital, The Fourth Military Medical University , Xi 'an 710038
【Abstract】 Objective To evaluate the stability and availability of the tree shrew model with hepatitis B. Methods 60 healthy tree shrews were injected either with 2.2.15 cells containing HBV or with confused patients serum with hepatitis B with various dose, by peritoneal injection, venous injection, and peritoneal combined with venous injection, respectively. The general clinical manifestations were observed daily. Serial blood samples were obtained and serum ALT levels, serum HBsAg, HBeAg and HBV-DNA were detected respectively. Every liver specimen obtained from 24 tree shrews was divided into two portions for immunohistochemical and light-microscopic examinations. Results Some of the tree shrews showed anepithymia, diarrhea temporally. HBV markers detection of all the experimental tree shrews were negative except 8 tree shrews in the group of venous injection, of which serum HBsAg, HBeAg and HBV-DNA were temporally positive at 2～3 week post injection. The liver nonspecific morphologic changes of tree shrews were not similar to those of patients with hepatitis B. HBsAg was not detected in the hepatic cells by immunoheistochemical staining using murine monoclonal antibody against HBs antigen. Conclusion All these findings suggest that tree shrews model with hepatitis B virus was not established succeedingly.
【Key words】 Hepatitis B virus Tree shrews Animal model
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