胡晓波 夏筠 李群 200025上海第二医科大学附属瑞金医院上海市伤骨科研究所 中华骨科杂志 1999 0 19 1


    关键词：胶原；膜；骨折 期刊 zhgkzz 0 实验研究 fur -->


    

【摘要】目的 本研究研制了一种诱导性人工骨膜。方法 用猪真皮提取纯化胶原，盐析法制备胶原膜，并用此吸附BMP,制备成胶原-BMP人工骨膜。用大鼠桡骨3mm长骨缺损作为骨折模型，设空白、单纯胶原膜、胶原-BMP膜包绕骨折段以及新鲜同种异体植骨共4组。术后0.5、1、2和3个月分批处死各组大鼠数只，处死前2天每只大鼠腹腔内注射钙黄绿素80mg/kg。处死后分别行X线和软X线摄片，血清BGP测定以及荧光显微镜观察。结果 胶原-BMP人工骨膜组的上述多项检测指标均表明其成骨效能明显优于其他各组。结论 作者认为该组成骨效果优良的原因是由于胶原膜吸附了BMP，同时具有骨诱导和骨引导的两种成骨作用，而仅胶原膜包绕骨折段的单纯骨引导效果并不理想。

The Preparation andExperimental Study of Inductive and Artificial Periosteum

HU Xiaobo, XIA Jun, LI Qun.

    Ruijin Hospital and Shanghai Institute of Traumatology and Orthopaedics, Shanghai 200025

【 Abstract 】 Objective Inthis study, a kind of inductive artificial periosteum was prepared.Methods The collagen of porcine dermis was separated and purified, and the collagen membrane wasmade with the method of the salt precipitation, and mixed with bone morphogenetic protein(BMP) to prepare artificial periosteum containing collagen and BMP. A defect of 3 mm inlength was produced at the radius of rats as fracture model, the rats were divided intofour groups including control,collagen membrane, collagen-BMP membrane and fresh allograft. These two kinds ofmembrane were respectively used to enclose the fractures. The rats were separatelyeuthanized at 0.5,1,2 and 3 months postoperatively and the serum were collected. Two daysbefore sacrifice,each rat was given intraperitoneal injection of calcein at the dose of 80mg/kg. X-ray and soft X-ray radiography of right forearmwere taken and the level of BGP in serum was measured. The histologic changes of thesamples were observed under fluorescent microscope.Results The resultsshowed that osteogenic ability in collagen-BMP membrane group was superior to that of other groups. Conclusion The collagen-BMPmembrane had the role of osteoinduction as well as guided bone regeneration.

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