陈岳祥 李石 张兴荣 张忠兵 孔宪涛 陈岳祥 李石 张兴荣 张忠兵 孔宪涛 上海第二军医大学长征医院 中华肝脏病杂志 1999 0 7 3


    关键词：牛磺酸 细胞外基质 前胶原 组织金属蛋白酶抑制因子-1 基因 期刊 zhgzbzz 0 实验研究 fur -->


    

摘要 目的 研究牛磺酸抗肝纤维化作用。 方法 用四氯化碳(CCl₄ )制备肝纤维化模型；免疫组化检测肝Ⅰ、Ⅲ、Ⅵ型胶原和透明质酸、层粘连素沉积；Northern blot杂交检测肝Ⅰ、Ⅲ型前胶原、组织金属蛋白酶抑制因子-1(TIMP-1) mRNA含量。 结果 CCl₄ 肝纤维化大鼠肝脏Ⅰ、Ⅲ、Ⅵ型胶原、透明质酸和层粘连素染色明显增多；Ⅰ、Ⅲ型前胶原、TIMP-1 mRNA显著增加。牛磺酸处理的大鼠，上述各指标的阳性染色明显减少，并可明显抑制Ⅰ、Ⅲ型前胶原基因表达，而对TIMP-1 mRNA却无明显影响。 结论 牛磺酸对CCl₄ 诱导的大鼠肝纤维化具有保护作用，可明显抑制纤维生成与沉积。提示它对防治肝纤维化有一定的临床意义。

    

Taurine inhibites deposition of extracellular matrix in experimental liver fibrosis in rats

CHEN Yuexiang, LI Shi, ZHANG Xingrong, et al.

    Department of Gastroenterology, Changzheng Hospital, Second Military Medical University, Shanghai 200003

Abstract Objective To study the effect of taurine on liver fibrosis and its mechanism. Methods Fibrosis was induced by the administration of carbon tertrachloride(CCl₄ ) in rats. Some of the animals were treated with taurine. The rats were killed after 12 weeks of CCl₄ treatment. Depositions of type Ⅰ, Ⅲ and Ⅳ collages, laminin and hyaluronic acid were studied in liver sections by immunohistochemical technique using specific antibody. The hepatic contents of type Ⅰ, Ⅲ procollage and tissue inhibitor of metalloproteinase-1(TIMP-Ⅰ) mRNA were determined by Northern blot hybridyzation. Results A significant elevations of hepatic collagen Ⅰ, Ⅲ, Ⅳ, laminin and hyaluronic acid were observed after 12 weeks of liver injury in animals without taurine treatment, and a definite increase in the amounts of hepatic type Ⅰ, Ⅲ procollagen and TIMP-1 mRNA was noted. Taurine prevented increases in type Ⅰ, Ⅲ procollagen mRNA expression as well as the accumulation of the collagens, laminin and hyaluronic acid in the liver. Conclusion The data indicate that taurine has a protective effect in CCl₄ -induced hepatic fibrosis. The results suggest taurine might be of potential value in clinical practice.

     ......