隋波 俞卫锋 张广明 王学敏 马永德 刘树孝 430030 武汉、同济医科大学附属同济医院肝病研究所 中华肝脏病杂志 1998 0 0 2


    关键词：肝硬化 胰岛 高胰岛素血症 高胰高血糖素血症 期刊 zhgzbzz 0 实验研究 fur -->


    

【摘要】 目的 探讨肝硬化大鼠胰岛α、β细胞功能及其与循环血液中胰岛素及胰高血糖素水平变化的关系。 方法 对采用CCL4制作的肝硬化鼠和正常鼠的胰岛进行分离和纯化，并对其体外生物学活性进行检测。 结果 肝硬化鼠循环血液中胰岛素及胰高血糖素水平明显高于正常对照组鼠(P<0.01)；其分离纯化的胰岛经体外培养48小时及在含糖基质中孵育2小时后释放胰岛素的量明显低于正常鼠(P<0.01)；而胰高血糖素释放量明显高于正常鼠(P<0.01)。 结论 ①肝硬化时循环血液中胰岛素及胰高血糖素水平增加；②胰岛α细胞分泌功能增加可能有助于肝硬化高胰高血糖素血症的发生；③肝硬化时，虽循环血液中胰岛素水平增加，但β细胞分泌功能低于正常。

    

CHANGES OF PANCREATICISLETS FUNCTION IN CIRRHOTIC RATS

Wang Tiancai, Li Shangao,Liang Kuohuan, et al. Institute of Liver Disease, Tongji Hospital, Tongji MedicalUniversity, Wuhan 400030

【Abstract】 Objective Thepurpose of this study was to find out changes of the pancreatic islets function incirrhotic rats. Methods Pancreatic islets from normal and cirrhotic rats were measured invitro in isolated islets. Results Rats with cirrhosis caused by carbon tetrachloride exhibitedhyperinsulinism and hyperglucagonism compared with control rats (P<0.01). Isolatedislets from cirrhotic rats showed impaired insulin secretion after culture for 48 hours orincubation in the presence of glucose for 2 hours. On the contrary, glucagon secretionincreased significantly. Conclusion Our data show that hyperglucagonism in portal hypertensive rats ispromoted by an enhanced a lpha-cell secretion, and the level of serum insulin is high.However, function of beta-cell secretion is low.

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