关键词:胎兔;外科技术
【摘要】 目的 建立简单有效的胎兔外科手术方法,使术后成活率达到80%以上。方法 在胎兔创伤愈合的研究中,采用在硫喷妥钠静脉麻醉下将胎兔胎体后1/2置于宫外或在宫内手术的方法;用37℃左右的生理盐水补充术中丢失的羊水;严密缝合羊膜。结果 手术后胎兔的成活率达到90%,胎兔发育正常。结论 此方法简单有效。
Techniques in fetal rabbit surgery Feng Guoping,Sun Guangci,Yang Xin,et al.Plastic Surgery Hospital of Chinese Academy of Medical Sciences,Beijing 100041
【Abstract】 Objective Experimental fetal surgery has been a popular way to study growth and development and wound healing.The rabbit continues to be a reasonable animal model.The key to success of the experiment lies in keeping the survival rate of the operated animal above 80 percent.In this paper a simple experimental method for fetus surgery in rabbits is described.Methods Intravenous thiopental sodium is used for general anesthesia.Surgery is performed inside the uterus through hysterotomy or with the caudal part of the fetus out of the uterus.Amniotic fluid is restituted by instillation of warm saline at 37℃.Special care is taken to suture the amnion.Results Using these techniques carefully,we have had 90 percent of success in fetal surgery experiment.Conclusion A technically simple procedure has been developed for fetal rabbit surgery.
【Key words】 Fetal rabbit Surgical technique
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