任新萍 高晖 王申五 王德炳 100044 北京医科大学血液病研究所 北京医科大学人民医院 中华肿瘤杂志 1998 0 0 3


    关键词：白血病/药物疗法 细胞凋亡 DNA，肿瘤/分析 流式细胞术 期刊 zhzlzz 0 基础研究 fur -->


    

【摘要 】 目的 了解急性白血病患者化疗过程中细胞凋亡的动态变化及意义。方法 采用细胞形态学、 DNA凝胶电泳、流式细胞计数仪研究 20例不同类型急性白血病化疗前后外周血变化。结果 化疗过程中外周血白细胞、幼稚细胞明显下降，但无凋亡细胞和 DNA Ladder形成， DNA含量分析也无亚二倍体峰出现。结论 化疗药物诱导细胞凋亡，但在血循环中很快被清除，故不易检测出凋亡细胞，体内研究细胞凋亡较体外更为复杂。

    A study on apoptotic cell death during chemotherapy of patients with acute leukemia Ren Xinping, Gao Hui, Wang Shenwu, et al. Institute of Hematology, People's Hospital, Beijing Medical University， Beijing 100044

    【 Abstract 】 Objective To investigate whether apoptosis occurs in vivo in patients with acute leukemia during induction chemotherapy and its significance. Methods Apoptotic effect on peripheral blood of 20 patients with different types of acute leukemia prior to and during routine chemotherapy was studied by cell morphology, DNA electrophoresis and FCM-DNA analysis. Results After chemotherapy, with falls in WBC and blast count, no apoptotic cells appeared on smears of peripheral blood, nor did ladder pattern of DNA on electrophoresis and subdiploid peak on FCM. Conclusion Although anticancer drugs have been shown to induce apoptosis in vitro, circulating apoptotic cells are hardly detected probably due to their rapid clearance from the circulation. It is more difficult to study apoptosis in vivo than in vitro.

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