关键词：粘附分子；整合素；细胞外基质；伤口愈合
【摘要】 目的 研究伤口愈合过程中，细胞外基质(Extracelluarmatrix, ECM)中粘附分子整合素β1 的表达与胶原合成之间的密切关系。方法 运用间接免疫胶体金标记技术，在电镜下观察了12例中厚取皮供区创面成纤维细胞表面粘附分子β1 整合素的表达及细胞超微结构的变化，并作了流式细胞仪检测。结果 中厚取皮供区创面成纤维细胞表面粘附分子整合素α5 及β1 亚单位的表达与对照组相比有显著差异；同时，前者细胞内富含有核糖体积聚的内质网，微管、微丝等细胞器的含量也较高。结论 上述结果揭示了在伤口愈合过程中，粘附分子整合素β1 的表达与胶原合成之间有重要的关系，为进一步探索粘附分子在伤口愈合中的作用打下了基础。
Expression of adhesionmolecules β 1 integrin on the surface of fibroblasts in wound healing
CHEN Minliang ,LIN Zihao,HONG Wei,et al.* Department ofPlastic and Reconstructive Surgery,Changzheng Hospital,the Second Military MedicalUniversity,Shanghai 200003
【 Abstract 】 Objective Toinvestigate the relationship between the expression of adhesion molecules integrin β 1 and the synthesis ofcollagen in wound healing.Methods In this study,integrin β 1 expression onfibroblasts derived from granulated wound and from normal skin was compared by thetechnique of immunoelectron microscope and flow cytometry.The differences ofultrastructure between the two groups of fibroblasts were also observed.Results Theresults showed that the expression of α 5 and β 1 subunits on fibroblasts derived from granulated woundwere higher than those from normal skin.Endoplasmic reticulum attached with more ribosomein the former cells was richer than that in the latter cells.Conclusion Theseresults suggest that the expression of adhesion molecules intergrin β 1 appears to be associated with the synthesisof collagen in wound healing.
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