于东宁 覃凤均 孙永华 程时 (于东宁、覃凤均、孙永华)100035 北京积水潭医院烧伤科；(程时)北京医科大学生物物理学系 中华整形烧伤外科杂志 1999 0 15 2


    关键词：烧伤；脂质过氧化；皮肤活性；金属硫蛋白 期刊 zhzxsswkzz 0 创面愈合 fur -->


    

【摘要】 目的 研究Wistar大鼠深Ⅱ度烧伤后皮肤活力的下降与脂质过氧化反应的关系。方法 以氧耗量、琥珀酸脱氢酶、Schiff's碱及创面愈合时间为指标，以金属硫蛋白作为保护剂外用于烫伤创面，分别采用空白对照及5×10^-6 mol/L，1×10^-5 mol/L两种浓度，测定伤后8，24，48h各项指标的变化。结果 皮肤氧耗量、琥珀酸脱氢酶活性下降，Schiffs碱含量明显升高。应用金属硫蛋白保护后，皮肤活性(氧耗及琥珀酸脱氢酶含量)均显著高于对照组(P<0.05或P<0.01)，同时Schiff’s碱含量下降(P<0.05或P<0.01)，创面愈合时间平均提前2天。结论 烫伤组织损伤与脂质过氧化反应有一定的关系，而金属硫蛋白有一定保护作用。

    Effects of metallothionein(MT) on burned skin of rats

YU Dongning, QIN Fengjun, SUNYonghua, et al.

    Department of Burns, Beijing Jishuitan Hospital, Beijing 100035

【 Abstract 】 Objective Toelucidate the relationship between skin viability and lipid peroxidation after deeppartial thickness burn injury.Methods Deep partial thickness burn wasproduced. Metallothionin (MT) in different concentrations (0,5×10^-6 mol/L,1×10^-5 mol/L)was topically applied. Oxygen consumption, succinate dehydrogenase(SDH), Schiff's basecontents and healing time of wound were determined in 24 Wistar rats. Specimens wereharvested at 8,24, and 48-hour postburn.Results Oxygen consumption andSDH value decreased and Schiff's base value increased after burn injury. With theapplication of MT, oxygen consumption and SDH value of burned skin were significantlyhigher compared with control group, and Schiff's base value was significantly lower(P<0.05 or P<0.01).The mean healing time of burned wound was two days less in two MTprotection groups. Conclusion MT may be beneficial in improving postburnskin viability by attenuating lipid peroxidation of burned tissue.

     ......