于明 李正 A. Hebra 于明、李正(110003 沈阳，中国医科大学附属第二医院小儿外科)；A. Hebra(美国费城儿童医院小儿外科) 中华小儿外科杂志 1999 0 20 1


    关键词：新生儿；肠细菌移位；缺血再灌注 期刊 zhxrwkzz 0 实验研究 fur -->


    

【摘要】 目的 了解新生儿肠缺血再灌注损伤对细菌移位的影响。方法 采用新生猪仔非阻塞性肠缺血再灌注模型，观察不同程度缺血再灌注所致肠道细菌移位及肠粘膜上皮损伤情况。结果 对照组无细菌移位；中度缺血仅有肠系膜淋巴结细菌移位；中度缺血再灌注、重度缺血、重度缺血再灌注均导致肠系膜淋巴结、外周血、肝、脾细菌移位。缺血再灌注组细菌移位数明显高于仅有缺血组(P<0.01)，重度缺血再灌注可见肠粘膜上皮损伤。结论 新生猪仔肠缺血再灌注可引起细菌移位及肠粘膜损伤，其严重程度与缺血严重程度及有无再灌注有关。这一病理改变应引起临床重视。

    Bacterial Translocation in Mesenteric Ischemia-reperfusion Injury in Newborn Piglets

YU Ming, LI Zheng, A. Hebra^* .

    Department of Pediatric Surgery, 2nd Affiliated Hospital, China Medical University,Shenyang 110003, ^* Department of Pediatric Surgery, Philadelphia Children'sHospital, USA.

【Abstract】 Objective Tostudy bacterial translocation during mesenteric ischemia-reperfusion injury.Methods Bacterialtranslocation (BT) and intestinal mucosal damage were studied in newborn piglets subjectedto various degress of non-occlusive ischemia-reperfusion injury.Results Thecontrol group (group Ⅰ) did not reveal any BT. 83% of piglets subjected to moderatedegree of ischemia (group Ⅱ) had BT to mesenteric lymph nodes. Piglets subjected tomoderate ischemia-reperfusion (group Ⅲ), severe ischemia alone (group Ⅳ) and severe ischemia-reperfusion (group Ⅴ) did not only grow positive culture in mesenteric lymphnodes, but also in peripheral blood, liver and spleen. The incidence of BT was higher in ischemia-perfusion groups than in the groups with ischemia alone (P<0.01). Intestinalmucosal damage was detected histologically in the severe ischemia-perfusion group.Conclusions Ischemia-reperfusioninjury induced BT and intestinal mucosal damage in newborn piglets. The extent of BT andthe presence of mucosal damage correlate with the severity of ischemia-reperfusion injury.

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