王练英 张志波 刘颖 李正 110003 中国医科大学第二临床学院小儿外科 中华小儿外科杂志 1999 0 20 1


    关键词：泄殖腔畸形；无肛畸形；动物模型 期刊 zhxrwkzz 0 实验研究 fur -->


    

【摘要】 目的 用乙烯硫脲诱发妊娠大白鼠产生泄殖腔畸形动物模型，观察其肛周肌肉病理改变。方法 取泄殖腔畸形鼠仔，正中矢状面连续切片，用Luzex-F图像分析仪测内、外括约肌及耻骨直肠肌面积。用肌球蛋白ATP酶染色，观察鼠仔盆底横纹肌复合体中Ⅰ、Ⅱ型肌纤维发育情况。结果 各种泄殖腔畸形内括约肌面积较正常鼠增大，后壁更明显(P<0.05)；肛门外括约肌面积较正常鼠明显小；低位泄殖腔畸形耻骨直肠肌面积较正常鼠增大，而中高位其面积较正常鼠小。泄殖腔畸形鼠仔盆底横纹肌复合体中Ⅰ型肌纤维占比例明显减少(P<0.05)。结论 泄殖腔畸形动物模型病理改变与人类极相似，为研究该畸形病理提供参考指标。

    Animal Model of Cloacal Anomalies

WANG Lianying, ZHANG Zhibo, LIU Ying, et al.

    Department of Pediatric Surgery, 2^nd Affiliated Hospital, China MedicalUniversity, Shenyang 110003

【Abstract】 Objective Tostudy the pathological changes of peri-anal musculature in Wistar rats withethylenethiourea induced cloacal anomalies.Methods Serial sagittalsections were performed on fetuses which developed cloacal anomalies after being exposedto ethylenethiourea in utero. Puborectalis, internal and external anal sphincters weremeasured with Luzes Fimage analyzer. The striated muscles of pelvic floor were stained fortype Ⅰ and type Ⅱ fibers.Results Of the cloacal anomalies group,the internal anal sphincter was significantly thicker than that of the normal, especiallyover the posterior aspect (P<0.05). The size of puborectalis muscle was larger than thatof normal in low-type anomalies, but smaller than normal in intermediate and high types ofanomalies. The proportion of type Ⅰ fibers in striated muscle complex was significantlylower (P<0.05).Conclusions The pathological changes in this animalmodel are similar to those seen in human beings. Hence the model provides a useful tool inresearch of cloacal anomalies.

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