杨高云 刘红涛 李世荫 姜学义 宋泉声 马大龙 杨高云 李世荫 姜学义(100083 北京医科大学第三医院皮肤科)；马大龙 刘红涛 宋泉声(北京医科大学分子免疫实验室) 中华皮肤科杂志 1999 0 32 1


    关键词：红斑狼疮；系统性；白细胞介素6；BXSB小鼠 期刊 zhpfkzz 0 论著 fur -->


    

【摘要 】 目的 为了进一步观察白介素6(IL-6)在系统性红斑狼疮(SLE)发病中的作用。方法 采用基因工程方法构建了真核表达载体pcDNA3-IL-6，并经体内和体外实验检测到被其转染的细胞高效表达IL-6和IL-6mRNA。先后2次(于第1、第20天)将这种质粒多点肌注于12只3.5月龄雄性BXSB狼疮小鼠，每次每只100μg，同时用等量空载体质粒(pcDNA3)肌注于10只同龄雄性BXSB狼疮小鼠作对照。结果 发现IL-6在该鼠体内的过表达导致其血清ANA滴度、尿蛋白水平和肾小球IgG、C3沉积较对照组明显增加，血清IL-6水平和肾小球IL-6表达高于对照组，并有2例小鼠脾脏明显增大。结论 证实了IL-6在SLE发病中的病理性作用，并提示阻止或抑制IL-6的产生或分泌可能有助于SLE的治疗。

Acceleration of Murine Lupus Disease Activity inBXSB Mice by IL-6 Gene Delivery

YANG Gaoyun, LIU Hongtao, LI Shiyin, et al.Department of Dermatology, Third Teaching Hospital, Beijing Medical University, Beijing 100083

【Abstract 】 Objective Tostudy the role of IL-6 in the pathogenesis of SLE. Methods Eukaryoticexpression vector pcDNA3-IL-6 encoding for IL-6 was constructed, and intramuscularlyinjected into 12 of 3.5-month-old-male BXSB mice at a dose of 100μg per mouse at aninterval of 20 days, and 10 male BXSB mice with same age were injected plasmid pcDNA3 atthe same time as control. Serum ANA titer and proteinuria were monitored every 10 days,serum level of IL-6, IgG, C3 deposition and IL-6 expression on kidney were detected at the40th day. Results IL-6 encoding plasmid had harmful effect on murineSLE with increased ANA level, proteinuria, IgG, C3 deposition and IL-6 expression onkidney compared with those of the control mice, but there was no difference on serum GPTlevels between the two groups. Conclusion These results support theconcept that excessive production of IL-6 might play an important role in the pathogenesisof SLE, which suggests that blocking or inhibiting the production or secretion of IL-6might be of therapeutic value in SLE.

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