曲伸 何小龙 何成 由振东 王成海 路长林 200433 上海，第二军医大学基础医学部神经生物教研室(曲伸系研究生，现在长海医院内分泌科) 中华神经科杂志 1999 0 32 1


    关键词：帕金森病；脑源性神经生长因子；基因疗法 期刊 zhsjkzz 0 论著 fur -->


    

【摘要】 目的 观察脑源性神经营养因子(BDNF)基因工程成肌细胞脑内纹状体移植对帕金森病大鼠的治疗作用。方法 建立逆转录病毒介导的BDNF表达质粒并转染成肌细胞，筛选阳性细胞进行脑内移植。结果 基因工程成肌细胞脑内移植可明显提高帕金森病大鼠黑质酪氨酸羟化酶(TH)阳性神经元的存活率，使纹状体多巴胺含量明显增加，动物的旋转行为改善约50%，并持续2个月之久。结论 脑源性神经营养因子基因工程成肌细胞脑内纹状体移植可明显改善动物的旋转行为并可促进黑质神经原的存活，为帕金森病的治疗提供了一种新的有效的治疗方法。

    Grafting myoblasts genetically engineered to produce BDNF in rats Parkinsondisease models QU Shen, HE Xiaolong, HE Cheng, et al. Department ofNeurobiology, The Second Military Medical University, Shanghai 200433.

    【Abstract】 Objective To observe the role of geneticallyengineered myoblast in the treatment of rat Parkinson disease models. Methods Genetically modified myoblasts to produce brain derived neurotrophic factors (BDNF)were established and grafted into the corpora striata of Parkinson disease modelsin rats.The behavior and pathological changes were observed and the Dopamine contents in thecorbora striata were measured with high performance liquid chromatography (HPLC). Results Theintracranial graft of genetically engineered myoblasts can promote the survival of TH-positive neurons in the nigra of Parkinson's rat and the content of Dopamine in thestriatum were increased significantly. The rotation behavior improved 50% after thetransplantation. Conclusion It is suggested that BDNF secreting myoblastscan promote the survival of the neuron and provide a new and effective method for thetreatment of Parkinson disease.

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