庄一义 汪俊军 李勇 210002 南京军区南京总医院全军医学检验中心 中华医学检验杂志 1999 0 0 2


    关键词：胆固醇酯类；载体蛋白质类 期刊 zhjyyxzz 0 论著摘要 fur -->


    

【摘要】 目的 建立一种新的分离纯化人血清胆固醇酯转运蛋白(CETP)的方法，为制备CETP单克隆抗体和CETP浓度测定提供物质基础。方法 制备无脂血清，用Butyl-Sepharose4FF、CM-Sepharose FF和Sephacryl S-200三步柱层析分离、纯化CETP。结果 纯化CETP经SDS-PAGE电泳鉴定呈单一区带，分子量65000，蛋白质含量0.325 mg/ml，与国外和作者研制CETP单抗的免疫斑点试验呈特异性反应，与纯化脂蛋白(a)、载脂蛋白(Apo)A1、ApoB、IgG、IgA、IgE、C₃ 、C₄ 抗体无免疫反应。结论 经三步柱层析获得了纯化CETP，得率约25%，活性每小时30 nmol/μg。

    Purification and identification of cholesteryl ester transfer protein ZHUANG Yiyi, WANG Junjun, LI Yong. Department Biochemistry, Nanjing General Amry Hospital,Nanjing 210002

    【Abstract】 Objective To separate and purify human serumcholeseryl ester transfer protein (CETP). Method Lipoprotein-freeserum, purification of CETP was completed by butyl-sepharose 4FF, CM-sepharose FF andsephacryl S-200 column chromatographic proceduces. Results Thispurified protein was confirmed as a human CETP by SDS-PAGE, specific immunoreaction withCETP monoclonal antibodies and CETP activity determination. The activity was 30 nmol/μg/h.The recovery rate was about 25%. Conclusion The method contributes topreparation of CETP monoclonal antibody and determination of CETP concentration.

     ......