谭耀驹 李一耕 张言斌 谢贝 萧婉云 陈虹 510095 广州市胸科医院肺部疾病研究所 中华结核和呼吸感染 1999 0 22 8


    关键词：聚合酶链反应；结核，肺；分支杆菌，结核 期刊 zhjhhhxgr 0 论著摘要 fur -->


    

【摘要 】 目的 探讨AmpliSensor-聚合酶链反应(AmpliSensor-PCR)定量检测外周血中结核分支杆菌DNA(TB-DNA)在肺结核的应用价值。方法 采用QIAamp和AcuPure法提取，制备全血中模板TB-DNA，应用AmpliSensor-PCR定量检测，并与IS6110-单管巢式聚合酶链反应(SN-PCR)作比较。结果200例肺结核患者的血液标本中，两种方法测得结核分支杆菌DNA的阳性率分别为60.5%、63.5%。85例非结核肺病患者(包括22名献血员)的阳性率分别为4.7%、8.2%。结论 AmpliSensor-PCR检测全血中的TB-DNA是一种敏感性、特异性较高的方法，其含量的变化对疾病的预后有一定的提示作用。

    

Quantitative detection of Mycobacterium tuberculosis DNA in peripheral blood from patients with pulmonary tuberculosis by AmpliSensor-PCR technique

TAN Yaoju, LI Yigeng, ZHANG Yanbin

Pulmonary Disease Institute, Guangzhou Chest Hospital, Guangzhou 510095

【Abstract 】 Objective To evaluate the clinical value of the quantitative detection of Mycobacterium tuberculosis DNA by Amplisensor-PCR in peripheral blood from patients with pulmonary tuberculosis. Methods The model of Mycobacterium tuberculosis DNA in blood was made by QIAamp and AcuPure methods, quantitative detection of Mycobacterium tuberculosis DNA by AmpliSensor-PCR technique and the results were compared with IS6110 single-tube nested PCR (SN-PCR). Results The peripheral blood from 200 patients with pulmonary tuberculosis were detected respectively by using AmpliSensor-PCR and IS6110 SN-PCR methods. The positive rates of Mycobacterium tuberculosis DNA were 60.5% and 63.5% respectively, and that from 85 patients with non-pulmonary tubeculosis were 4.7%, 8.2%. Conclusions AmpliSensor-PCR method showed higher sensitivity and specificity in detecting the Mycobacterium tubeculosis DNA from peripheral blood. The variation of the TB-DNA may be useful in evaluating the prognosis of the patients.

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