【摘要】 目的 观察脂多糖(LPS)致肺血管内巨噬细胞(PIM)核因子κB(NF-κB)的活化及抗炎药物地塞米松(DEX)和阿斯匹林(ASA)对NF-κB的影响。方法 用改良法分离、培养猪PIM，设正常对照、LPS刺激、DEX和ASA干预组，共4组。用凝胶电泳迁移率改变分析(EMSA)法和放射免疫分析(RIA)法，分别检测PIM核提取物NF-κB的活性和细胞培养上清肿瘤坏死因子α(TNF-α)的含量。结果 LPS刺激组NF-κB活性于刺激后0.5～4小时、TNF-α含量于刺激后1～2小时高于刺激前和正常对照组(P<0.01)；二者在刺激后1小时呈显著正相关(r=0.991，P<0.01)。DEX组和ASA组NF-κB活性、TNF-α含量虽较刺激前和正常对照组有所升高，但均显著低于LPS组(P<0.01)。结论 LPS可诱导PIMNF-κB激活，并进而导致TNF-α的基因转录和表达增加；DEX和ASA可通过抑制NF-κB的活化而减少TNF-α的释放。

    

The effects ofLipopolysaccharide and anti-inflammatory drugs on nuclear factor-κ B in pulmonary intravascular macrophage

CAI Enqi, CHEN Zhengtang, WU Weiling. Instisuteof Respiratory Medicine of PLA, Xinqiao Hospital, Third Military Medical University,Chongqing 400037

【Abstract】 Objective To investigate activation ofnuclear factor-κB(NF-κB) in pulmonary intravascular macrophage (PIM) stimulated withlipopolysaccharide (LPS) and effects of anti-inflammatory drugs dexamethasone (DEX) andaspirin (ASA) on the process. Methods PIMs isolated from three healthypigs were cultured and divided into four groups: Control group; LPS- stimulated group; DEX- or ASA-treated group. The NF-κB activity of nuclear protein extract from the PIMsand the concentration of tumor necrosis factor α (TNF-α) in the supernatant weremeasured by electrophoretic mobility shift assay (EMSA) and radioimmunoassay (RIA),respectively. Results The activity of NF-κB significantly increasedfrom 0.5 hr to 4 hr after LPS stimulation (P<0.01); The level of TNF-α in thesupernatant elevated markedly from 1 hr to 2 hr after LPS stimulation (P<0.01);positive correlation was found between the NF-κB activity and the TNF-α concentation at1 hr after LPS stimulation (r=0.991, P<0.01). Compared with LPS-stimulated group, bothNF-κB activity and TNF-α concentration were significantly lowered in DEX-or ASA-treatedgroups (P<0.01). Conclusions LPS might activate NF-κB in the PIMs,and induce the increase of transcription and expression of TNF-α gene; Both DEX and ASAcould inhibit the activation of NF-κB and reduce the release of TNF-α.

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