杨国华 李志海 杨春珠 孟庆刚 杨国华(150001 哈尔滨医科大学第一临床医学院急诊创伤外科)；孟庆刚(150001 哈尔滨医科大学第一临床医学院急诊创伤外科)；杨春华(急诊内科)；李志海(整形外科) 中华手外科杂志 2000 0 16 1


    关键词：再灌注损伤；钙通道阻滞药；脂质过氧化作用；外科皮瓣 期刊 zhswkzz 0 基础研究 fur -->


    

摘 要：目的 探讨钙离子拮抗剂对大鼠腹部皮瓣缺血再灌注损伤的保护作用。方法 建立大鼠腹壁下动脉岛状皮瓣缺血再灌注的模型，检测应用钙离子拮抗剂维拉帕米(Verapamil，Ver)和汉防己甲素(Tetrandcin，Tet)前后，皮瓣脂肪组织中超氧化物歧化酶(SOD)活性、谷胱甘肽过氧化酶(GSH- Px)活性和丙二醛(MDA〕含量的变化。结果 Ver和Tet可以明显地提高皮瓣的SOD活性和GSH - Px活性,并可显著降低 MDA的含量。结论 钙离子拮抗剂能减轻大鼠腹部皮瓣缺血再灌注损伤的脂质过氧化作用。

Study of theantioxidation effect of calcium antagonist in rat abdominal skin flap

YANG Guohua LI Zhihai YANG Chunzhu,et al.

    ( The First ClinicalMedical College of Harbin Medical University 150001,China)

Abstract： Objective To observe the protective effect of calcium antagonist onthe ischemia - reperfusion injury in rat abdominal skin flap.Methods 33 SD rats wererandomized into three groups. The abdominal island flap based on the inferior epigastricvessels was used as the ischemia - reperfusion model. In the control group, normal salinewas given intraperitoneally for 3 consecutive days preoperatively, while Verapamil (Ver)and Tetrandcin (Tet) were given in the other two groups respectively. Samples from theflap were harvested during ischemia and reperfusion periods to measure the changes ofsuperoxide dismutase ( SOD) activity,glutathione peroxidase ( GSH - Px) activity and malonyl dialdehyde ( MDA) content. Results SOD activity and GSH - Px activity were much higher in theVer and Tet groups than in the control group, while the MDA content was remarkablydecreased in these two groups.Conclusions Calcium antagonist, Verapamil and Tetrandcin, plays animportant role in oxygen free radical scavenging and in reducing lipid peroxidation.

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