【摘要 】 目的 探讨端粒酶活性在肺癌支气管肺泡灌洗液(BALF)细胞中的表达与肺癌发生机制的关系。方法 应用银染-多聚酶链方法对肺癌患者和支气管、肺良性疾病患者各30例的BALF细胞端粒酶活性的表达进行检测。结果 肺癌患者BALF细胞端粒酶活性83%阳性表达，支气管、肺良性疾病BALF细胞表达均阴性(P<0.001)。同一肺癌患者患侧肺端粒酶活性在BALF细胞83%表达，健侧肺阳性表达27%。结论 端粒酶活性是肺癌诊断的标志物之一，与肺癌的发生发展密切相关。

【Abstract 】 Objective Toinvestigate the telomerase activity in BALF cells from patients with lung cancer. Method The silver staining and PCR techniqne were used to detect thetelomerase activity in BALF cells from 30 patients with lung cancer and 30 patients withchronic bronchitis or benign pulmonary diseases. Result The telomerase activity in BALF cells was positivein all the patients with lung cancer (25/30, 83%), and in none of the patients withchronic bronchitis or benign pulmonary diseases (0/30, 0%). The difference was highlysignificant (P<0.001). In the same patient with lung cancer, the positive rate oftelomerase activity was 83% in BALF cells from the diseased lung, but only 27% in BALFcells from the normal lung (P<0.001). Conclusion The telomerase activity may be used as a diagnostic marker for lungcancer. There is a correlation between telomerase activity and the development of lungcancer.

     【Key words 】 Lung neoplasm Telomeraseactivity Bronchoalveolar lavage fluids

人类与衰老的研究中 ......