【摘要 】 目的 探讨外周血单个核细胞中结核分支杆菌DNA检测在肺结核诊断中的价值。方法 采用改良Triton X-100法分离、制备单个核细胞中模板DNA，聚合酶链反应(PCR)扩增结核分支杆菌240 bp基因片段，同时分析了影响PCR结果的有关因素。结果 89例肺结核患者的血标本、84例肺结核患者的痰标本中，结核分支杆菌DNA阳性率分别为73%和57%；84例肺结核患者外周血、痰标本配对检测总阳性率可达87%。30例非结核患者的血标本PCR阳性率为10%。结论 改良TritonX-100法处理标本后用PCR检测外周血单个核细胞中结核分支杆菌DNA具有快速、简便、灵敏、特异等优点，结合痰标本检测，可提高肺结核诊断的敏感性和准确性，对肺结核的诊断具有重要的参考价值。

Tang Shaohua^* , Bao Xiaoou, Zheng Tingdi. ^* Departmentof Heredity, Second Hospital of Wenzhen, Wenzhen 325000

【Abstract 】 Objective To evaluate the clinical value of detection of Mycobacteriumtuberculosis DNA (MTB-DNA) in peripheral blood mononuclear cells (PBMC) for diagnosis ofpulmonary tuberculosis.Method Polymerase chain reaction (PCR) technique was used to amplify geneof 240 bp DNA fragment prepared by Triton X-100 method, and some factors affecting PCRresult were also analysed.Result In blood samples of 89 patients with pulmonary tuberculosis and insputum samples of 84 patients with pulmonary tuberculosis, the positive rates of PCR were73% and 57% respectively, and the total positive rate of combinative detection of bloodand sputum samples was 87% in 84 pulmonary tuberculosis patients. In 30 non-tuberculosispatients, 3 showed MTB-DNA positive. Conclusion PCR technique prepared by Triton X-100 method is rapid, simple,specific, and sensitive for detection of MTB-DNA in PBMC, and its sensitivity and accuracycould be increased in combination with sputum MTB examination. Detection of MTB-DNA inPMBC is of value in diagnosis of pulmonary tuberculosis.

     ......