【摘要 】 目的 评价间质巨噬细胞(IM)对成纤维细胞增殖的影响。方法 在成纤维细胞培养液中分别加入肺纤维化大鼠肺泡巨噬细胞(AM)、IM培养上清培养。结果 实验组第1天两种巨噬细胞的促增殖作用即开始升高,第7天达高峰(AM332%,IM 342%，P<0.01)；而IM和AM培养上清中肿瘤坏死因子α(TNF-α)、血小板源生长因子(PDGF)与成纤维细胞增殖活性水平成显著相关(r=0.657,0.713,P<0.01)。结论 不仅AM,而且IM也能释放促成纤维细胞体外增殖的细胞因子。由于IM与成纤维细胞紧密相邻,对纤维化的形成可能更具有意义。

Studies on pulmonary fibrosis inducedby interstitial macrophages Lü Changjun, Zhao Hongwen, Hou Xianming, et al. Department of Respiratory, The AffiliatedHospital, BinZhou Medical College, ShanDong 256603

【Abstract 】 Objective Toevaluate the role of interstitial macrophages (IMs) in modulating the proliferativecapacity of fibroblast(FB). Method IMsisolated by collagenase IA digestion of disaggreted lung tissues from bleomycin-treatedrats were studied in this regard.The culture supernatants of alveolar macrophages(AMs) andof IMs were added to culture media of FBs. Result The releases of growth factors by the two sub-populations ofmacrophage were already elevated on 1st day following bleomycin instillation, and peakedon 7th day at a level nearly 3-fold above that of control groups(IM 342%, AM 332% ......