【摘要】 目的 克隆ATM全长cDNA及含特异功能域的cDNA片段，寻找与ATM相互作用的蛋白，分析ATM在DNA损伤监视中的分子机理。方法 利用长片段PCR扩增法，从人外周血来源的cDNA库中扩增ATM cDNA; 利用酵母双杂交系统筛选人外周血来源的cDNA库中与ATM相互作用的蛋白。结果 经重叠PCR扩增到ATM全长cDNA，并筛选到数条与ATMPI3K激酶区相 互作用的cDNA，并对其序列进行分析。 结论 ATM通过与多种蛋白相互作用，传递DNA损伤信号。

Participation of ATM incellular response to DNA

    damage induce d by ionizing radiation

MENG Xiangbing,SONG Yi,MAO Jianping,etal. Department of Biochemistry,Institute of Radiation Medicine,Beijing 100850,China

【Abstract】 Objective To clone ATMfull length cDNA and cDNA frag ments containing some functional domains and to identifyproteins that interact with ATM and mediate DNA damage signal transduction in cellularresponse to DNA damage. Methods ATM cDNA was amplified from Marthom^TM -ReadycDNA k it of human leukocytes by LD-PCR.ATM-interacting proteins were screened by yeasttwo hybrid system. Results ATM full-length cDNA and cDNA fragmen ts containing PI3K kinasedomain,leucine zipper and proline rich region were ampli fied from human cDNAs.Severalcandidate clones that interacted with ATM PI3K dom ain were identified. Conclusion ATMmediates DNA damage signal transduction by interac ting with many proteins.

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