刘黎军 朱家恺 肖建德 刘黎军 朱家恺 中山医科大学第一附属医院显微外科(广州，510080)；肖建德 深圳市红十字会医院骨二科；刘黎军 现在深圳市红十字医院骨二科(广东深圳，518029) 中国修复重建外科杂志 1999 0 13 5


    关键词：雪旺细胞源神经营养因子；运动神经元； 一氧化氮合酶； 一氧化氮 期刊 zgxfcjwkzz 0 生长因子的研究与应用 fur -->


    

【摘 要】 目的 研究雪旺细胞源神经营养因子(SDNF)对臂丛神经根性撕脱伤所致前角运动神经元死亡的保护作用。方法 选成年SD大鼠，制成颈_6、7 神经根性撕脱伤动物模型，损伤处定期应用SDNF，3周后观察损伤侧脊髓前角运动神经元的成活率和形态学变化，以及一氧化氮合酶(NOS)表达的情况。结果 对照组68.6%的脊髓前角运动神经元死亡，成活神经元胞体明显萎缩，同时表达NOS神经元增多；实验组脊髓前角运动神经元的死亡率较对照组降低35%，成活神经元胞体无萎缩，表达NOS神经元未见增多。结论 SDNF对臂丛神经根性撕脱伤所致运动神经元死亡有明显的保护作用，一氧化氮在臂丛神经根性撕脱伤所致运动神经元死亡中起一定作用。

RESCUE OF MOTONEURON FROMBRACHIAL PLEXUS NERVE ROOT AVULSION INDUCED CELL DEATH BY SCHWANN CELL DERIVEDNEUROTROPHIC FACTOR

LIU Li-jun,ZHU Jia-kai, XIAOJian-de.

Department of Microsurgery, FirstAffiliated Hospital,Sun Yat-sen University of Medical Sciences.GuangzhouGuangdong,P.R.China 510080

【 Abstract】 Objective To study the protective effects of Schwann cell derivedneurotrophic factor (SDNF) on motoneurons of spinal anterior horn from spinal rootavulsion induced cell death.Methods Twenty SD rats were made the animal model of C_6,7 spinal root avulsion induced motoneuron degeneration,and SDNF was applied at the lesionsite of spinal cord once a week. After three weeks, the C_6,7 spinal region wasdissected out for motoneuron count, morphological analysis and nitric oxide synthase (NOS)enzyme histochemistry.Results 68.6% motoneurons of spinal anterior horn death wereoccurred after 3 weeks following surgery, the size of survivors was significantly atrophyand NOS positive neurons increased. However, in animals which received SDNF treatment, thedeath of motoneurons was significantly decreased, the atro-phy of surviving motoneuronswas prevented, and expression of NOS was inhibited.Conclusion SDNF can prevent the death ofmotoneurons following spinal root avulsion.Nitric oxide may play a role in these injuryinduced motoneuron death.

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