关键词：增生性瘢痕；瘢痕疙瘩；细胞培养
摘 要 为探讨不同瘢痕在体外培养情况下，其成纤维细胞生物学特性，切取正常皮肤、增生性瘢痕和瘢痕疙瘩分别行体外成纤维细胞原代和传代培养，观察成纤维细胞形态学和生长动力学变化。结果发现，三类细胞的形态学方面没有明显差异，但瘢痕疙瘩成纤维细胞排列更加紊乱，极性消失，有交叉重叠现象。在接种相同的细胞密度和相同的培养条件下，其细胞生长率、分裂指数、克隆形成及DNA含量无明显差别。认为，三类细胞在体外培养状态下，其形态学和生长动力学无显著差异，且具有相似的生物学特性。
OBSERVATION OFCICATRICIAL FIBROBLASTS IN CULTURE AND ITS BIOLOGICAL PROPERTIES /Huang Yong, Ren Linsen, Qin Ying. Department of Plastic Surgery, First University Hospital, West ChinaUniversity of Medical Sciences. Chengdu, P.R.China 610041
Abstract In order to study the biological properties of fibroblasts isolatedfrom different tissues. The fibroblasts from normal skin, hypertrophic scar and keloidwere cultured, respectively, in vitro, and their morphologies and growth kinetics werecompared. The results revealed that although fibroblasts in keloid were irregularlyarranged, crisscross and overlapping with loss of polarization, there was no significantdifference in the 3 groups so far the cellular morphology of fibroblast itself, cellulargrowth curve, cellular mitotic index, cloning efficiency and DNA content provided thosecultures were in the same cellular density and culture conditions. It was concluded thatfibroblasts isolated from culture of normal skin, hypertrophic scar and keloid in vitroshowed no significant difference in morphology and growth kinetics, on the contrary, theirbiological behaviors were quite similar.
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