【摘 要】 目的 观察经皮自体骨髓移植在骨缺损瘢痕组织内的成骨作用 ，探索治疗骨不连的新途径。方法 选健康家兔18只，建立双侧桡 骨中段骨及骨膜缺损1cm模型，6周后，实验侧(右)骨缺损区经皮注射自体红骨髓2ml，对 照侧(左)骨缺损区经皮注射自身外周血2ml。在不同时间内进行X线片、组织学检查及新生 组织钙、磷含量测定。临床应用经皮自体骨髓移植治疗骨不连15例，其中腕舟骨不连7例， 肱 骨不连3例，股骨不连2例，胫骨不连3例。骨不连时间为7～48个月，平均13个月。结果 实验侧骨缺损区在骨髓注射后不同时间的X线片、组织学检查均显示新 骨 形成逐渐增多，钙、磷含量均高于对照侧。对照侧无成骨现象。临床应用的15例中，13例在 5～9个月骨折愈合，2例未愈合。结论 经皮自体骨髓移植在骨缺损 瘢痕组织内有成骨作用，在临床上可作为治疗骨不连的方法，尤其适用于骨不连部位软组织 条件差、不具备开放植骨的病例。

EXPERIMENTAL STUDY AND CLINICAL APPLICATION ON OSTEOGENESIS OF P ERCUTANEOUS AUTOGENOUS BONE MARROW GRAFTING IN BONE DEFECTS

LIANG Yu-tian,ZHANG Bo-xun,LU Shi-bi,et al.

    Department of Orthopedic Surge ry,General Hospital of PLA. Beijing, P.R.China 100853

【Abstract】 Objective To observe the osteogenesis of percutaneous autogenous bone marrow grafting in cicatricial bone defect,to seek a good method for treating fracture nonunion.[ WT5 ”HZ〗Methods Eighteen rabbits were adopted in this study.1 cm bone defect model was made in each side of radius, 6 weeks later,2 ml autogenous bon e marrow was injected in the right radial bone defect as experimental group,2 ml autogenous peripheral blood in the left side as control group. X-ray featu res,histologic changes, Ca and P content in the site of bone defect were studied in various times.Also 15 patients were treated clinically for the nonunion frac t ure,the average time from nonunion to bone marrow grafting was 13 months.Results In experimental group, the increasing new bone tissue we re observed in X-ray and histologic examination.While in control group, no oste ogenesis was observed.Ca and P content of experimental group was higher than tha t of control group.For the 15 patients ,13 cases healed in 5～9 months,2 cases f ailed.Conclusion Percutaneous autogenous bone marrow gra fting is capable of osteog enesis in the cicatricial bone defects.It can be used in nonunion cases which ar e not fit for operation of bone grafting because of poor condition of the skin.

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