摘 要：目的： 探讨利用癌症患者自体成纤维细胞作为抗原提呈细胞，诱导抗肿瘤的细胞毒T淋巴细胞(CTL)反应的可能性。方法 ：制备阳离子脂质体，并包裹大肠癌相关抗原CA-Hb3。制备自体成纤维细胞、肿瘤细胞和外周血淋巴细胞(PBL)。借助脂质体将CA-Hb3抗原导入成纤维细胞浆内，采用此细胞抗原周期刺激法，体外诱导抗原特异性CTL反应。制备自体肿瘤的荷瘤鼠模型，并进行过继治疗实验。结果： 成功地诱导了抗原特异性CTL反应，获得的CTL在体外对亲本肿瘤细胞有显著的细胞毒活性，而不杀伤异体的抗原靶细胞，其培养上清中IFN-γ和TNF-α分泌水平增高。结论： 大肠癌患者自体成纤维细胞经CA-Hb3抗原修饰后，可以作为有效的抗原提呈细胞，诱导抗大肠癌的抗原特异性CTL反应。

    分类号：R392.12 R735.3

Fibroblasts as efficient antigen-presenting cells in induction of specific CTL responses

Abstract ： Objective ： To characterize and develop a potential of activatingtumor-specific cytotoxic T lymphocyte(CTL) responses with autologous fibroblasts of cancerpatients.Methods: CA-Hb3, a colorectal cancer-associated antigen,wasencapsulated within cationic liposomes and introduced into the cytoplasmas of theautologous fibroblasts which were applied to activate T cells from the autologousperipheral blood lymphocytes(PBL). Adoptive therapy experiment in the tumor-bearing modelswas performed.Results: The antigen-specific CTL responses weresuccessfully observed.The CTLs could lysed the autologous tumor cells other thanallogeneic antigen targets in vitro with elevated levels of IFN- γ and TNF- α in the culture supernants.Conclusion: Theautologous fibroblasts modified with CA-Hb3 antigen could be as efficientantigen-presenting cells to induce specific CTL responses against colorectal cancer.

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